Viral Genome Methods

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Molecular biology and genetics techniques now dominate viral research in attempts to cure diseases such as AIDS. Viral Genome Methods is a practical guide to the newest molecular techniques, providing step-by-step protocols to be used in the laboratory. Recognized authorities and pioneers in viral research pass on their expertise to you.

Author(s): Kenneth W. Adolph
Publisher: CRC Press
Year: 1996

Language: English
Pages: 314
City: Boca Raton

Cover
Half Title
Title
Copyright
Preface
The Editor
Contributors
Contents
Section I. Retroviruses/HIV/RNA Viruses
Chapter 1. Retrovirus-Mediated Gene Transfer
Chapter 2. Methods for Studying Genetic Variation of the Human Immunodeficiency Virus (HIV)
Chapter 3. Methods for HIV/SIV Gene Analysis
Chapter 4. Analysis of Human Immunodeficiency Virus Reverse Transcriptase Function
Chapter 5. Tat Activation Studies: Use of a Reporter Cell Line
Chapter 6. Characterization of HIV Nef Cell-Specific Effects
Chapter 7. Detection, Quantitation, and Characterization of Human Retroviruses
Chapter 8. Quantitative Methods to Monitor Viral Load in Simian Immunodeficiency Virus Infections
Chapter 9. Using Molecular Genetics to Probe the Immune Response to Vesicular Stomatitis Virus
Section II. DNA Viruses
Chapter 10. Transient Genetic Approaches to Studying Multifactor Processes in Complex DNA Viruses
Chapter 11. Epstein-Barr Virus: Gene Expression and Regulation
Chapter 12. Functional Analysis of the Herpes Simplex Virus DNA Polymerase
Chapter 13. Molecular Genetics of Adeno-Associated Virus
Chapter 14. Reconstitution of Adenovirus DNA Replication with Purified Replication Proteins
Section III. Index