Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed. * Methods presented for ease of use and reasdy adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)
Author(s): Kenneth W. Adolph (Eds.)
Series: Methods in Molecular Genetics 7
Edition: 1
Publisher: Elsevier, Academic Press
Year: 1995
Language: English
Pages: 3-416
Content:
Contributors to volume 7
Pages ix-xiii
Preface
Page xv
Kenneth W. Adolph
Methods in molecular genetics
Page xvii
[1] Adeno-associated virus: Integrating vectors for human gene therapy Original Research Article
Pages 3-12
A.W. Skulimowski, R.J. Samulski
[2] Techniques for human adenovirus vector construction and characterization Original Research Article
Pages 13-30
Mary Hitt, Andrew J. Bett, Christina L. Addison, Ludvik Prevec, Frank L. Graham
[3] Construction and isolation of recombinant adenoviruses with gene replacements Original Research Article
Pages 31-44
David J. Spector, Lorna A. Samaniego
[4] Construction and use of recombinant vaccinia virus vectors Original Research Article
Pages 45-64
Douglas Grosenbach, Dennis E. Hruby
[5] Epstein-barr viral plasmid vectors and their amplifiable derivatives Original Research Article
Pages 65-86
Ann L. Kirchmaier, Tim A. Bloss, David Mackey, Bill Sugden
[6] Methods to study epstein-barr virus and p53 status in human cells Original Research Article
Pages 89-100
Alison J. Sinclair, Paul J. Farrell
[7] The SV40 minichromosome Original Research Article
Pages 101-113
Claudia Gruss, Rolf Knippers
[8] Site-specific integration of multigenic shuttle plasmids into the herpes simplex virus type 1 genome using a cell-free cre-lox recombination system Original Research Article
Pages 114-130
Siyamak Rasty, William F. Goins, Joseph C. Glorioso
[9] Strategies for studying transcription regulation of neurotropic viruses in the central nervous system: Lesson from JC virus Original Research Article
Pages 131-151
Jay Rappaport, Douglas A. Kerr, Kamel Khalili
[10] Analysis of the nuclear DNA-binding activity in cells overexpressing hepatitis B viral X and 3′ truncated S transactivators Original Research Article
Pages 152-166
Volker Schlüter, Wolfgang H. Caselmann
[11] Investigations on virus-host interactions: An abortive system Original Research Article
Pages 167-192
Christiane Zock, Walter Doerfler
[12] Quantitation of virus stocks produced from cloned human immunodeficiency virus DNA Original Research Article
Pages 195-208
Carol A. Deminie, Michael Emerman
[13] Quantitation of HIV-1 RNA in plasma or serum samples Original Research Article
Pages 209-217
V. Natarajan, N.P. Salzman
[14] Detection of cell fusion mediated by the envelopes of human retroviruses by transactivation of a reporter gene Original Research Article
Pages 218-236
Tatjana Dragic, Uriel Hazan, Marc Alizon
[15] Assembly of recombinant retroviral gag precursors into pseudovirions in the baculovirus-insect cell expression system Original Research Article
Pages 237-253
Gregory J. Tobin, Jane K. Battles, Lynn Rasmussen, Matthew A. Gonda
[16] Retrovirus reverse transcription and integration Original Research Article
Pages 254-266
Jonathan Leis, Ashok Aiyar
[17] In Vitro binding and transcription assays using the human T-cell leukemia virus type I tax protein Original Research Article
Pages 267-279
Mark G. Anderson, William S. Dynan, Maura-Ann H. Matthews
[18] Polymerase chain reaction-based techniques for utilizing retroviruses as cell lineage markers Original Research Article
Pages 280-295
Christopher A. Walsh
[19] New methods to study poliovirus assembly and encapsidation of genomic RNA Original Research Article
Pages 299-314
Casey D. Morrow, Donna C. Porter, David C. Ansardi
[20] Molecular studies of hepatitis delta virus RNAs Original Research Article
Pages 315-328
Tie-Bo Fu, Ting-Ting Wu, David Lazinski, John Taylor
[21] Systems to express recombinant RNA molecules by the influenza A virus polymerase in vivo Original Research Article
Pages 329-342
Ignacio Mena, Carmen Albó, Agustín Portela, Susana de la Luna, Javier Martín, Beatriz Perales, Amelia Nieto, Juan Ortín
[22] Analysis of membrane association in vivo and targeting of viral proteins in polarized epithelial cells Original Research Article
Pages 343-358
Debi P. Nayak, Amitabha Kundu, Christopher M. Sanderson
[23] Cloning of viral double-stranded RNA genomes by single primer amplification Original Research Article
Pages 359-372
Paul R. Lambden, Ian N. Clarke
[24] Using the RNA-capture assay to assess the RNA-binding activity of viral proteins Original Research Article
Pages 373-387
John T. Patton, Jian Hua
[25] Protein RNA-binding activity measured by northwestern blot analysis: The interferon-inducible RNA-dependent protein kinase PKR Original Research Article
Pages 388-403
Stephen J. McCormack, Charles E. Samuel
Erratum
Page 404
Index
Pages 405-416
