Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive “manual” in a field that has become as broad and deep as cardiovascular medicine, this volume of “Methods in Molecular Medicine” covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.
Author(s): Nair Sreejayan, Jun Ren
Series: Methods in Molecular Medicine
Edition: 1
Publisher: Humana Press
Year: 2008
Language: English
Pages: 372
Cover......Page 1
Frontmatter......Page 2
Rat Carotid Artery Balloon Injury Model......Page 13
Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model......Page 43
Plaque Rupture Model in Mice......Page 79
Immunostaining of Mouse Atherosclerotic Lesions......Page 88
Surgical Animal Model of Ventricular Hypertrophy......Page 106
Animal Models of Hypertension......Page 116
Rat Models of Cardiac Insulin Resistance......Page 123
The Isolated, Perfused Pseudo-Working Heart Model......Page 154
Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice......Page 160
Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress......Page 171
Detection and Quantification of Apoptosis in the Vasculature......Page 188
Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction......Page 203
Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells......Page 217
Evaluation of Endothelial Function by In Vivo Microscopy......Page 231
Haptotaxis of Endothelial Cell Migration Under Flow......Page 242
Isolation, Culture, and Functional Analysis of Adult Mouse Cardiomyocytes......Page 256
Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes......Page 268
Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia......Page 276
Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells......Page 287
Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues......Page 297
Assessment of Protein Glycoxidation in Ventricular Tissues......Page 316
Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes......Page 332
Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research......Page 342
Stem Cell Therapy in the Heart and Vasculature......Page 357
Index......Page 368