Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as well as ultrasensitive enzyme immunoassays. Ultrasensitive and Rapid Enzyme Immunoassay, with its clear presentation and up-to-date information, will be essential to scientists in pharmaceutical companies and in applied research.
Author(s): P.C. van der Vliet (Eds.)
Series: Laboratory techniques in biochemistry and molecular biology 27
Edition: 1
Publisher: Elsevier
Year: 1999
Language: English
Commentary: 66610
Pages: ii-v, 1-334
City: Amsterdam; New York
Content:
Edited by
Page ii
Copyright page
Page iv
Preface
Page v
Eiji Ishikawa
Chapter 1 Introduction: classification of immunoassays
Pages 1-3
Chapter 2 History of ultrasensitive enzyme immunoassay
Pages 5-6
Chapter 3 Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoassays
Pages 7-16
Chapter 4 Methods to minimize effects of factors limiting the sensitivity
Pages 17-77
Chapter 5 Principle of the immune complex transfer enzyme immunoassay
Pages 79-95
Chapter 6 Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations
Pages 97-140
Chapter 7 Ultrasensitive and rapid enzyme immunoassay
Pages 141-176
Chapter 8 Protocol of immune complex transfer enzyme immunoassay
Pages 177-191
Chapter 9 Ultrasensitive immunoassay for haptens
Pages 193-210
Chapter 10 Properties of the reagents for enzyme-labeling
Pages 211-221
Chapter 11 Enzyme-labeling of antibodies
Pages 223-248
Chapter 12 Preparation and affinity-purification of IgG fragments
Pages 249-259
Chapter 13 Protocol for enzyme-labeling of antibodies
Pages 261-275
Chapter 14 Protocol for labeling avidin with 2,4-dinitrophenyl groups and β-D-galactosidase
Pages 277-282
Chapter 15 Enzyme-labeling of antigens
Pages 283-287
Chapter 16 Protocol for enzyme-labeling of antigens
Pages 289-302
Chapter 17 Assays of enzymes
Pages 303-309
References
Pages 311-325
Subject Index
Pages 327-334
