Based on an international conference in France in 1990, this book draws together experts in ultra low dosage pharmacology, with topics covering experimental pharmacology, biophysics, biochemistry and toxicology, cell biology and clinical pharmacology.
Author(s): C. Doutremepuich
Edition: 1
Year: 1991
Language: English
Pages: 162
BOOK COVER......Page 1
HALF-TITLE......Page 2
TITLE......Page 3
COPYRIGHT......Page 4
CONTENTS......Page 5
PREFACE......Page 7
I EXPERIMENTAL PHARMACOLOGY......Page 8
METHODS AND MATERIALS......Page 9
RESULTS......Page 10
METHODS AND MATERIALS......Page 11
RESULTS......Page 12
CONCLUSIONS......Page 14
REFERENCES......Page 15
THE EFFECTS OF SOME REGULATORY PEPTIDES IN FEMTOMOLAR AND LOWER CONCENTRATIONS OF LYMPHATIC VESSELS (LVs)......Page 16
REFERENCES......Page 19
INTRODUCTION......Page 21
Experimental design......Page 22
RESULTS......Page 23
DISCUSSION......Page 25
REFERENCES......Page 28
INTRODUCTION......Page 29
Culture Medium......Page 30
Flow Cytometry......Page 31
I. Hierarchy of Sensitivity and Resistance of Tumor Cells to Various Cytotoxic Systems......Page 32
s Factor (TNF-a) and Chemotherapic Drugs .........Page 33
A. Effect on Tumor Cells Sensitive to TNF and drug......Page 34
B. Effect on Tumor Cells Resistant to TNF and Sensitive to Drug......Page 35
C. Effect on Tumor Cells Sensitive to TNF and Resistant to Drug......Page 36
D. Effect on Tumor Cells that are Resistant to both TNF and Drug......Page 37
III. Examination on the Possible Mechanisms Involved in Overcoming Drug Resistance by TNF and drug......Page 39
DISCUSSION......Page 40
REFERENCE......Page 42
Drugs......Page 43
Preparation of vascular fragments......Page 44
Statistics......Page 45
Curve amplitude......Page 46
Latency time......Page 47
REFERENCE......Page 48
II BIOPHYSICS......Page 50
INTRODUCTION......Page 51
COMPUTATIONAL STRATEGY......Page 52
Electrostatic Potentials......Page 53
Electrostatic Similarities......Page 58
Comparison with crystallographic results......Page 59
ACKNOWLEDGEMENTS......Page 60
REFERENCES......Page 61
INTRODUCTION......Page 62
MATERIALS AND METHODS......Page 63
RESULTS......Page 65
DISCUSSION......Page 69
REFERENCES......Page 70
III BIOCHEMISTRY—TOXICOLOGY......Page 72
INTRODUCTION......Page 73
Corpuscular Derangement......Page 74
DISCUSSION......Page 77
REFERENCES......Page 78
METHODS......Page 79
RESULTS AND CONCLUSION......Page 80
REFERENCES......Page 81
IV CELL BIOLOGY......Page 82
INTRODUCTION......Page 83
Materials......Page 84
Dilutions of stimulants and inhibitors......Page 85
Assay of superoxide production and of adhesion......Page 86
Optimal experimental conditions and kinetics......Page 88
Dose-response curves......Page 90
Printing by endotoxin......Page 92
Effects of inhibitors......Page 95
DISCUSSION......Page 96
ACKNOWLEDGEMENTS......Page 99
REFERENCES......Page 100
Isolation of Peripheral Mononuclear Cells......Page 102
RESULTS......Page 103
DISCUSSION......Page 105
REFERENCES......Page 107
INTRODUCTION......Page 108
A/ Preparation of the Basophil Suspension......Page 109
D/ Basophil Staining and Counting......Page 110
E/ Processing of the Data......Page 111
B/ Effect of Histamine and Histamine Dilution on Basophil Degranulation......Page 112
C/ Effect of Histaminase on the Inhibition of Basophil Degranulation by Dilutions of Histamine......Page 113
E/ Time-effect phenomenon......Page 114
tesimal dilution .........Page 115
DISCUSSION......Page 116
REFERENCES......Page 117
I/ Samples Selection......Page 119
B/ Basophil Degranulation......Page 120
RESULTS......Page 121
DISCUSSION......Page 122
REFERENCES......Page 123
V CLINICAL PHARMACOLOGY......Page 124
INTRODUCTION......Page 125
RESULTS......Page 126
DISCUSSION......Page 127
REFERENCES......Page 128
MATERIAL AND METHODS......Page 129
Experimental design......Page 130
Coagulation tests......Page 131
Platelet aggregation on PRP......Page 132
DISCUSSION......Page 133
REFERENCE......Page 134
Index......Page 136
