This volume explores disease diagnosis and therapy in developing theranostics. The chapters in this book are divided into four parts and cover various techniques used in studying bioengineering, molecular diagnostics, in vivo imaging, and imaging-guided therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Theranostics: Methods and Protocols is a valuable tool for novice and expert clinicians, researchers, and students working in this multidisciplinary field.
Author(s): Batra Jyotsna
Series: Methods in Molecular Biology, 2054
Publisher: Humana Press
Year: 2019
Language: English
Pages: 294
Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis (Eleonora Peerani, Juliana B. Candido, Daniela Loessner)....Pages 3-21
Bioengineered Microtissue Models of the Human Bone Metastatic Microenvironment: A Novel In Vitro Theranostics Platform for Cancer Research (Nathalie Bock)....Pages 23-57
Real-Time and 3D Quantification of Cancer Cell Dynamics: Exploiting a Bioengineered Human Bone Metastatic Microtissue (Nathalie Bock, Joan Röhl)....Pages 59-77
Front Matter ....Pages 79-79
Exosomes Extraction and Identification (Xiaoxin Wu, Salah Ali A. Showiheen, Antonia Rujia Sun, Ross Crawford, Yin Xiao, Xinzhan Mao et al.)....Pages 81-91
Profiling MicroRNA Markers in Plasma: Looking into Better Approaches and Recommendations (Farhana Matin, Jyotsna Batra)....Pages 93-103
Isolation and Quantification of MicroRNAs from Human Saliva (Sri Ram Arunachalam, Kai Dun Tang, Chamindie Punyadeera)....Pages 105-114
The Isolation and Analysis of Circulating Tumor Cells (Tianyu Guo, Elzbieta Stankiewicz, Xueying Mao, Yong-Jie Lu)....Pages 115-128
The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples Using Spiral Microfluidic Technology (Arutha Kulasinghe, Majid Ebrahimi Warkiani, Chamindie Punyadeera)....Pages 129-136
Pharmacogenetics: Role of Single Nucleotide Polymorphisms (Emrah Yucesan, Nur Ozten)....Pages 137-145
EGFR Mutation Analysis in Non-small Cell Lung Carcinoma from Tissue Samples Using the Fully Automated Idylla™ qPCR System (Simon Heeke, Paul Hofman)....Pages 147-155
Front Matter ....Pages 157-157
Subcellular Localization of MicroRNAs by MicroRNA In Situ Hybridization (miR-ISH) (Harley Rose Robinson, Michelle Mei Chih Hill, Alexandre Santos Cristino)....Pages 159-169
Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies (Laura V. Croft, Jaimie A. Mulders, Derek J. Richard, Kenneth O’Byrne)....Pages 171-183
Luminescent Porous Silicon Nanoparticles for Continuous Wave and Time-Gated Photoluminescence Imaging (Tushar Kumeria, Zhi Qu, Amirali Popat, Tariq Altalhi, Abel Santos)....Pages 185-198
Front Matter ....Pages 199-199
Nucleic Acid Aptamers as Emerging Tools for Diagnostics and Theranostics (Ruchi Mutreja, Shahnawaz Ahmad Baba, Naveen Kumar Navani)....Pages 201-221
Aptamer Selection for Detecting Molecular Target Using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology (Kimberly D. Stewart, Weihong Tan, Jong Y. Park)....Pages 223-241
Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes (Elzbieta Stankiewicz, Tianyu Guo, Xueying Mao, Yong-Jie Lu)....Pages 243-261
Upconversion Nanoparticle-Based Strategy for Crossing the Blood-Brain Barrier to Treat the Central Nervous System Disease (Libing Fu, Roger Chung, Bingyang Shi)....Pages 263-282
Genetically Engineered Plasma Membrane Nanovesicles for Cancer-Targeted Nanotheranostics (Pengfei Zhang, Hu Chen, Jingyi Liu, Gang Liu)....Pages 283-294
Back Matter ....Pages 295-296
