The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology

Bringing together the latest information into one easily accessible resource, The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology explores the diverse tools and technologies used to study synaptic processes. Written by world-renowned leaders in the field, the book delineates newly developed techniques, methods, and conceptual advances used for studying neurotransmitter receptors and other synaptic proteins. A broad array of molecular, biochemical, imaging, and electrophysiological approaches for studying the biology of synapses are described. Specific topics include the use of proteomics to study synaptic protein complexes, the development of phosphorylation state specific antibodies, post-genomic tools applied to the study of synapses and RNA interference in neurons. In addition, several chapters focus on methods for gene and protein delivery into neuronal tissue. The use of biochemical, electrophysiological and optical tagging techniques to study the movement and membrane trafficking of neurotransmitter receptors in the membrane of live nerve cells are also discussed. To complement these approaches, the application of state of the art approaches for achieving long-term alterations in the genetic complement of neurons in vivo using viral vectors or homologous recombination of ES cells are also described. This book provides researchers interested in studying the development and plasticity of synapses and the regulation of ion channels and receptors with detailed information on cutting edge techniques that can be applied to this area. The information is useful to both new and experienced synaptic physiologists as well as the wider neuroscience community.