Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.
Author(s): Sung Ho Yoon, Haeyoung Jeong (auth.), Prof.Dr. Sang Yup Lee (eds.)
Edition: 1
Publisher: Springer Netherlands
Year: 2009
Language: English
Pages: 462
Tags: Biochemistry, general; Microbiology; Microbial Genetics and Genomics; Biotechnology; Applied Microbiology
Front Matter....Pages I-XXI
Genomics, Biological Features, and Biotechnological Applications of Escherichia coli B: “Is B for better?!”....Pages 1-17
Escherichia coli Genome Engineering and Minimization forthe Construction of a Bioengine....Pages 19-40
Multi-Omics Data-Driven Systems Biology of E. coli ....Pages 41-57
A Medium-Throughput Structural Proteomics Approach Applied to the Genome of E. coli ....Pages 59-86
Resources for Escherichia coli Systems Biology....Pages 87-97
The Multiple Scientific Disciplines Served by EcoCyc....Pages 99-112
Analysis of E. coli Network....Pages 113-132
Sensory Transduction Network of E. coli ....Pages 133-148
Genome-Scale Reconstruction, Modeling, and Simulation of E. coli℉s Metabolic Network....Pages 149-176
Kinetic Modeling of E. coli Enzymes: Integration of in vitro Experimental Data....Pages 177-207
Dynamic Modeling of the Central Metabolism of E. coli – Linking Metabolite and Regulatory Networks....Pages 209-235
Genome-Scale Models and the Genetic Basis for E. coli Adaptation....Pages 237-256
Organisation of Complex Escherichia coli Promoters....Pages 257-271
Plasmid Regulation and Systems-Level Effects on Escherichia coli Metabolism....Pages 273-294
Systems-Level Analysis of Protein Quality in Inclusion Body-Forming Escherichia coli Cells....Pages 295-326
Mechanistic Challenges and Engineering Applications of Protein Export in E. coli ....Pages 327-349
Engineering E. coli Central Metabolism for Enhanced Primary Metabolite Production....Pages 351-376
Glucose and Acetate Metabolism in E. coli – System Level Analysis and Biotechnological Applications in Protein Production Processes....Pages 377-400
Performance Characteristics for Sensors and Circuits Used to Program E. coli ....Pages 401-439
Systems Metabolic Engineering of E. coli ....Pages 441-453
Back Matter....Pages 455-462
