The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis.
For the first time, a team of recognized international experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field.
This second of two volumes covers applications of fluorescence for bioanalysis and biomedical diagnostics.
Author(s): Andrew Dixon, Thomas Heinlein, Ralf Wolleschensky (auth.), Ute Resch-Genger (eds.)
Series: Springer Series on Fluorescence 6
Edition: 1
Publisher: Springer-Verlag Berlin Heidelberg
Year: 2008
Language: English
Pages: 564
Tags: Analytical Chemistry; Molecular Medicine; Medical Biochemistry; Measurement Science, Instrumentation; Characterization and Evaluation of Materials
Front Matter....Pages I-XVI
Front Matter....Pages 1-2
Quantitative Fluorescence Microscopy: Considerations and Controls....Pages 3-24
Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standards....Pages 25-54
Front Matter....Pages 55-88
State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy....Pages 89-116
Single Molecule Spectroscopy: Instrumentation and Multiparameter Detection....Pages 117-142
Front Matter....Pages 143-144
DNA Microarrays: Applications, Future Trends, and the Need for Standardization....Pages 145-197
Protein Arrays and Fluorescence Detection: Applications and Limitations....Pages 199-212
Front Matter....Pages 213-214
Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations....Pages 215-237
Flow Cytometry Quality Assurance....Pages 239-263
Approaches to Quantitation in Flow Cytometry....Pages 265-282
Front Matter....Pages 283-304
Immunoassays: Basic Concepts, Physical Chemistry and Validation....Pages 305-306
Particle-Based Assays: Applications and Unresolved Issues....Pages 307-342
Advances in Fluorescence Enzyme Detection Methods....Pages 343-370
Front Matter....Pages 371-398
Quantitative Real-Time PCR: Fluorescent Probe Options and Issues....Pages 399-400
Front Matter....Pages 401-428
Front Matter....Pages 429-447
Back Matter....Pages 449-468
....Pages 469-485
