Content: Protein release from chemically permeabilized Escherichia coli / David J. Hettwer and Henry Y. Wang --
Structured and simple models of enzymatic lysis and disruption of yeast cells / J.B. Hunter and J.A. Asenjo --
Dual hollow-fiber bioreactor for aerobic whole-cell immobilization / Ho Nam Chang, Bong Hyun Chung, and In Ho Kim --
A membrane reactor for simultaneous production of anaerobic single-cell protein and methane / R.K. Finn and E. Ercoli --
Membrane processes in the separation, purification, and concentration of bioactive compounds from fermentation broths / Enrico Drioli --
Liquid emulsion membranes and their applications in biochemical separations / M.P. Thien, T.A. Hatton, and D.I.C. Wang --
Use of aqueous two-phase systems for recovery and purification in biotechnology / Bo Mattiasson and Rajni Kaul --
Recovery of proteins from polyethylene glycol-water solution by salt partition / G.B. Dove and G. Mitra --
Modeling of precipitation phenomena in protein recovery / C.E. Glatz and R.R. Fisher --
Process considerations for scale-up of liquid chromatography and electrophoresis / S.R. Rudge and M.R. Ladisch --
Mathematical modeling of bioproduct adsorption using immobilized affinity adsorbents / Somesh C. Nigam and Henry Y. Wang --
High-resolution, high-yield continuous-flow electrophoresis / William A. Gobie and Cornelius F. Ivory --
Scale-up of isoelectric focusing / Milan Bier --
Large-scale gel chromatography : assessment of utility for purification of protein products from microbial sources / James J. Kelley, George Y. Wang, and Henry Y. Wang --
Electron paramagnetic resonance spectroscopy studies of immobilized monoclonal antibody structure and function / Erik J. Fernandez, Forrest B. Fernandez, Roger B. Jagoda, and Douglas S. Clark.
Author(s): Juan A. Asenjo and Juan Hong (Eds.)
Series: ACS Symposium Series 314
Publisher: American Chemical Society
Year: 1986
Language: English
Pages: 231
City: Washington, DC
Title Page
......Page 1
Half Title Page
......Page 3
Copyright
......Page 4
ACS Symposium Series......Page 5
FOREWORD......Page 6
PdftkEmptyString......Page 0
PREFACE......Page 7
1 Protein Release from Chemically Permeabilized Escherichia coli......Page 9
Methods......Page 10
Results and Discussion......Page 11
Literature Cited......Page 15
2 Structured and Simple Models of Enzymatic Lysis and Disruption of Yeast Cells......Page 16
Model Background......Page 18
Models......Page 20
Special variables.......Page 23
Wall hydrolysis equations.......Page 25
Results......Page 28
Applications......Page 31
Literature Cited......Page 36
3 Dual Hollow-Fiber Bioreactor for Aerobic Whole- Cell Immobilization......Page 39
Materials and Methods......Page 40
Results and Discussion......Page 42
Literature Cited......Page 49
4 A Membrane Reactor for Simultaneous Production of Anaerobic Single-Cell Protein and Methane......Page 50
Anaerobic SCP Process......Page 51
Literature Cited......Page 56
5 Membrane Processes in the Separation, Purification, and Concentration of Bioactive Compounds from Fermentation Broths......Page 57
Membrane Systems in Downstream Processes......Page 58
Enzyme Membrane Reactors......Page 63
Enzyme Membranes......Page 64
Literature Cited......Page 71
Concept......Page 72
Applications......Page 75
Experimental System......Page 76
Results......Page 77
Acknowledgments......Page 80
Literature Cited......Page 82
7 Use of Aqueous Two-Phase Systems for Recovery and Purification in Biotechnology......Page 83
Extractive bioconversions in aqueous two-phase systems......Page 84
Purification of proteins by partition in aqueous two-phase systems......Page 90
Removal of polymers......Page 92
Economy......Page 94
Conclusion......Page 95
Literature cited......Page 96
8 Recovery of Proteins from Polyethylene Glycol-Water Solution by Salt Partition......Page 98
Properties and Applications of Aqueous Systems......Page 99
Results......Page 102
Applications......Page 109
Literature Cited......Page 112
9 Modeling of Precipitation Phenomena in Protein Recovery......Page 114
Protein Precipitation......Page 115
Models of the particle size distribution......Page 117
Precipitate Behavior......Page 121
Literature Cited......Page 124
10 Process Considerations for Scale-up of Liquid Chromatography and Electrophoresis......Page 126
The Capacity Factor in Chromatography......Page 127
Scale-Up Considerations......Page 130
Theoretical Considerations In Size Exclusion Chromatography......Page 133
Electrophoresis......Page 139
Plate Height and Resolution Concepts in Electrophoresis......Page 145
Summary......Page 150
Nomenclature......Page 151
Greek......Page 153
Literature Cited......Page 154
11 Mathematical Modeling of Bioproduct Adsorption Using Immobilized Affinity Adsorbents......Page 157
Theory......Page 158
Simulation Studies......Page 162
Legend of Symbols......Page 169
Literature Cited......Page 171
12 High-Resolution, High-Yield Continuous-Flow Electrophoresis......Page 173
Continuous Flow Electrophoresis......Page 174
Recycle CFE (RCFE)......Page 175
RCFE Model......Page 177
Discussion......Page 182
Conclusion......Page 185
Legend of Symbols......Page 187
Literature Cited......Page 188
Isoelectric Focusing......Page 189
Illustrative Fractionation Results......Page 191
Formation of pH Gradients......Page 193
Center for Separation Science......Page 195
Literature Cited......Page 196
14 Large-Scale Gel Chromatography Assessment of Utility for Purification of Protein Products from Microbial Sources......Page 197
Model Development......Page 200
Strategy for Utilizing the Model......Page 205
Cost Analysis......Page 208
Legend of Symbols......Page 210
Literature Cited......Page 211
15 Electron Paramagnetic Resonance Spectroscopy Studies of Immobilized Monoclonal Antibody Structure and Function......Page 212
Materials and Methods - EPR Spectroscopy......Page 214
Results and Discussion......Page 216
Concluding Remarks......Page 219
Literature Cited......Page 220
Author Index......Page 221
A......Page 222
C
......Page 223
D
......Page 224
E
......Page 225
H
......Page 226
L
......Page 227
O
......Page 228
P
......Page 229
S
......Page 230
Y
......Page 231
