This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.
Author(s): Peixuan Guo, Farzin Haque
Series: Methods in Molecular Biology 1297
Publisher: Humana Press
Year: 2015
Language: English
Pages: xii+239
Tags: Специальные дисциплины;Наноматериалы и нанотехнологии;Нанобиотехнология;
1 Overview of Methods in RNA Nanotechnology: Synthesis, Purification, and Characterization of RNA Nanoparticles - Farzin Haque and Peixuan Guo
2 Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly - Christophe Lavelle, Florent Busi, and Véronique Arluison
3 Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM - Kyung Duk Koh, Hsiang-Chih Chiu, Elisa Riedo, and Francesca Storici
4 Silver Nanoclusters for RNA Nanotechnology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies - Kirill A. Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, and Bruce A. Shapiro
5 Large Scale Purification of RNA Nanoparticles by Preparative Ultracentrifugation - Daniel L. Jasinski, Chad T. Schwartz, Farzin Haque, and Peixuan Guo
6 HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single-Nucleotide Resolution - Zhen Huang, Chi-Yen Lin, William Jaremko, and Li Niu
7 Using RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Prokaryotic and Eukaryotic Cells - Hui Zhang, Fengmei Pi, Dan Shu, Mario Vieweger, and Peixuan Guo
8 Fluorescence Labeling of Short RNA by Oxidation at the 3′-End - Chen Qiu, Wang-Yi Liu, and Yong-Zhen Xu
9 Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases - Piotr Rychahou, Yi Shu, Farzin Haque, Jiyao Hu, Peixuan Guo, and B. Mark Evers
10 Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor - Tae Jin Lee, Farzin Haque, Mario Vieweger, Ji Young Yoo, Balveen Kaur, Peixuan Guo, and Carlo M. Croce
11 Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens - Laetitia Evadé, Eric Dausse, Said Taouji, Emilie Daguerre, Eric Chevet, and Jean-Jacques Toulmé
12 Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer–siRNA Nanoparticles Via the Sticky Bridge - Jiehua Zhou, John J. Rossi, and Ka To Shum
13 A High-Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells - John M. Dean and Robert K. DeLong
14 Design, Assembly, and Evaluation of RNA–Protein Nanostructures - Hirohisa Ohno, Eriko Osada, and Hirohide Saito
15 Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq - Baochang Fan, Peng Ni, and C. Cheng Kao
16 Mapping Protein–RNA Interactions by RCAP, RNA-CrossLinking and Peptide Fingerprinting - Robert C. Vaughan and C. Cheng Kao
