Author(s): David R. Engelke John J. Rossi
Series: Methods in Enzymology 392
Edition: 1
Publisher: Elsevier, Academic Press
Year: 2005
Language: English
Pages: 496
0121827976......Page 1
Copyright......Page 5
Table of Contents......Page 6
Contributors to Volume 392......Page 9
Methods in Enzymology......Page 14
[1] Transgene-Induced RNA Interference as a Tool for Plant Functional Genomics......Page 37
[2] Constructs and Methods for Hairpin RNA-Mediated Gene Silencing in Plants......Page 60
[3] RNA Interference in Caenorhabditis elegans......Page 72
[4] High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells......Page 91
[5] Mechanistic Insights Aid Computational Short Interfering RNA Design......Page 109
[6] Novel Methods for Expressing RNA Interference in Human Cells......Page 133
[7] Delivery of Small Interfering RNA to Mammalian Cells in Culture by Using Cationic Lipid/Polymer-Based Transfection Reagents......Page 148
[8] Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization......Page 161
[9] Viral Delivery of Recombinant Short Hairpin RNAs......Page 181
[10] Targeting Cellular Genes with PCR Cassettes Expressing Short Interfering RNAs......Page 209
[11] Use of Short Hairpin RNA Expression Vectors to Study Mammalian Neural Development......Page 222
[12] Analysis of T-Cell Development by Using Short Interfering RNA to Knock Down Protein Expression......Page 235
[13] Lentiviral Vector Delivery of Recombinant Small Interfering RNA Expression Cassettes......Page 254
[14] Paradigms for Conditional Expression of RNA Interference Molecules for Use Against Viral Targets......Page 263
[15] High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways......Page 278
[16] RNA Interference In Vivo: Toward Synthetic Small Inhibitory RNA-Based Therapeutics......Page 314
[17] Labeling and Characterization of Small RNAs Associated with the RNA Interference Effector Complex RITS......Page 333
[18] RNA Interference Spreading in C. elegans......Page 344
[19] Human Dicer: Purification, Properties, and Interaction with PAZ PIWI Domain Proteins......Page 352
[20] Delivery of siRNA and siRNA Expression Constructs to Adult Mammals by Hydrodynamic Intravascular Injection......Page 372
[21] Analysis of Short Interfering RNA Function in RNA Interference by Using Drosophila Embryo Extracts and Schneider Cells......Page 387
[22] Use of RNA Polymerase II to Transcribe Artificial MicroRNAs......Page 407
[23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression......Page 417
[24] Simple, Robust Strategies for Generating DNA-Directed RNA Interference Constructs......Page 441
Author Index......Page 456
Subject Index......Page 479
