RNA Biochemistry and Biotechnology describes various aspects of nucleic acid and protein structure, mainly RNA structure and proteins, interacting with specific RNA species. Papers deal with DNA protein interactions, telomerase, aminoacyl-tRNA synthetases, elongation factor Tu, DNA repair, RNA structure, NMR technology, RNA aptamer interaction of biological macromolecules with metal ions. Two papers deal with theoretical aspects of RNA structure production and computer modelling. Many papers describe the possibility of commercial application of RNA biotechnology. One article discusses the impact of direct democracy on basic science supporting biotechnology.
Readership: Advanced graduate students, Ph.D. students and young scientists as well as specialists in the field.
Author(s): J. Barciszewski, B. F. C. Clark (auth.), Jan Barciszewski, Brian F. C. Clark (eds.)
Series: NATO Science Series 70
Edition: 1
Publisher: Springer Netherlands
Year: 1999
Language: English
Pages: 370
Tags: Biochemistry, general; Thoracic Surgery; Virology
Front Matter....Pages i-xv
Why RNA?....Pages 1-9
Algorithms and Thermodynamics for RNA Secondary Structure Prediction: A Practical Guide....Pages 11-43
Recurrent RNA Motifs....Pages 45-61
Towards the 3D Structure of 5s rRNA....Pages 63-71
Structure and Dynamics of Adenosine Loops in RNA Bulge Duplexes. RNA Hydration at the Bulge Site....Pages 73-87
The Structure and Function of the Ribozyme Rnase P RNA is Dictated by Magnesium(II) Ions....Pages 89-109
Metal Ion - Induced Cleavages in Probing of RNA Structure....Pages 111-121
Protein-DNA Recognition....Pages 123-126
Specific Interaction Between Damaged Bases in DNA and Repair Enzymes....Pages 127-138
Telomeric DNA Recognition....Pages 139-142
Recognition of One tRNA by Two Classes of Aminoacyl-tRNA Synthetase....Pages 143-148
Functional Structures of Class-I Aminoacyl-tRNA Synthetases....Pages 149-158
Aminoacylation of tRNA Induces a Conformational Switch on the 3’-Terminal Ribose....Pages 159-168
Point Mutants of Elongation Factor Tu from E. Coli Impaired in Binding Aminoacyl-tRNA....Pages 169-193
RNA Structure and RNA-Protein Recognition During Regulation of Eukaryotic Gene Expression....Pages 195-216
RNA-Aptamers for Studying RNA Protein Interactions....Pages 217-228
Probing of Ribonucleoprotein Complexes with Site-Specifically Derivatized RNAs....Pages 229-240
The IRE Model for Families of RNA Structures....Pages 241-247
Functional Analysis of RNA Signals in the HIV-1 Genome by Forced Evolution....Pages 249-275
Interaction of Native RNAs with Tat Peptides....Pages 277-290
Biogenesis, Structure and Function of Small Nucleolar RNAs....Pages 291-302
RNA Structure Modules with Trinucleotide Repeat Motifs....Pages 303-314
Phosphorothioate Oligonucleotides as Aptamers of Retroviral Reverse Transcriptases....Pages 315-324
Oxathiaphospholane Method of the Stereocontrolled Synthesis of Phosphorothioate Analogues of Oligonucleotides....Pages 325-334
Towards Improved Applications of Cell-Free Protein Biosynthesis - The Influence of mRNA Structure and Suppressor tRNAS on the Efficiency of the System....Pages 335-345
RNA on the Web....Pages 347-352
How Risky is Direct Democracy for Basic Science?....Pages 353-361
Back Matter....Pages 363-370
