The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Highlights * Cloning * Expression systems * Signal transduction * Structure-function techniques * Antireceptor antibodies * Regulation * 3-D receptor modeling and computational probing
Author(s): Stuart C. Sealfon (Eds.)
Series: Methods in Neurosciences 25
Publisher: Academic Press
Year: 1995
Language: English
Pages: 3-519
Content:
Contributors to volume 25
Pages ix-xii
Preface
Page xiii
Stuart C. Sealfon
Methods in neurosciences
Pages xv-xvi
[1] Approaches to receptor cloning Original Research Article
Pages 3-8
Stuart C. Sealfon
[2] Purification of receptors Original Research Article
Pages 9-43
David I. Schuster, Randall B. Murphy
[3] Protein and peptide microsequencing: Applications in neuroscience and receptor research Original Research Article
Pages 44-66
Michael J. Walsh
[4] Xenopus oocytes: A system for expression cloning and structure-function studies of ion channels and receptors Original Research Article
Pages 67-89
Roger D. Zühlke, Hui-Juan Zhang, Rolf H. Joho
[5] Cloning of G protein-coupled opioid receptors using degenerate PCR and low-stringency homology screening Original Research Article
Pages 90-104
David K. Grandy, Qun-Yong Zhou, Claudia Bouvier, Carmen Saez, James R. Bunzow
[6] Hybrid arrest screening in oocytes Original Research Article
Pages 105-125
Manami Tsutsumi, Boaz Gillo
[7] Receptor cloning: High-throughput sequencing of cDNA tags for identification of novel genes Original Research Article
Pages 126-141
Ewen F. Kirkness, J. Craig Venter
[8] Ligand binding and second-messenger assays for cloned Gq/G11-coupled neuropeptide receptors: The GnRH receptor Original Research Article
Pages 145-162
R.P. Millar, J. Davidson, I. Wakefield, C. Flanagan
[9] Receptor expression in mammalian cells Original Research Article
Pages 163-174
Rachael L. Neve, Kim A. Neve
[10] Baculovirus expression of receptors and channels Original Research Article
Pages 175-200
Michael Cascio
[11] Real-time measurements of receptor activity: Applications of microphysiometric techniques to receptor biology Original Research Article
Pages 201-224
John A. Salon, John C. Owicki
[12] Regulation of Na+-H+ exchange by G protein-coupled receptors Original Research Article
Pages 225-241
Kim A. Neve, Mary P. Rosser, Diane L. Barber
[13] Receptor-activated tyrosine phosphatases: Activity assays and molecular cloning Original Research Article
Pages 242-260
Philip J.S. Stork, Anita Misra-Press, Ming-Gui Pan
[14] Site-directed mutagenesis Original Research Article
Pages 263-277
Tung Ming Fong, Mari R. Candelore, Catherine D. Strader
[15] Receptor chimers Original Research Article
Pages 278-301
Sankuratri Suryanarayana, Brian K. Kobilka
[16] Mapping G protein coupling domains by site-specific peptides Original Research Article
Pages 302-321
Dieter Palm, Gerald Münch, Daria Malek
[17] Synthesis and expression of synthetic genes: Applications to structure-function studies of receptors Original Research Article
Pages 322-344
Cynthia J.L. Carruthers, Thomas P. Sakmar
[18] Use of receptors expressed in Escherichia coli to study autoimmunity against G protein-coupled membrane proteins Original Research Article
Pages 345-365
Johan Hoebeke, Jean-Gérard Guillet, A. Donny Strosberg
[19] Integrated methods for the construction of three-dimensional models and computational probing of structure-function relations in G protein-coupled receptors Original Research Article
Pages 366-428
Juan A. Ballesteros, Harel Weinstein
[20] Anti-fusion protein antibodies specific for receptor subtypes Original Research Article
Pages 431-454
Brian J. Ciliax, Craig Heilman, Sharon Edmunds, Steven M. Hersch, Allan I. Levey
[21] Development of antireceptor antibodies using synthetic peptides Original Research Article
Pages 455-469
Marjorie A. Ariano, David R. Sibley
[22] Receptor mRNA measurement by multiplex nuclease protection assay Original Research Article
Pages 470-491
Moshe Jakubowski
[23] Antisense DNA/RNA-based strategies to analysis of signal transduction via G proteins Original Research Article
Pages 492-509
Meiling Shih, Christopher M. Moxham, Craig C. Malbon
Index
Pages 511-519
