5th Edition. - Elsevier, 2011. - 616 p. - (Methods in Cell Biology, Volume 102) Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.Contents
Recent advances in Cytometry Instrumentation, Probes and Methods
Down-sizing cytometry to "micro" dimension
Droplet Microfluidics for High-throughput Analysis of Cells and Particles
Parallel Imaging Microfluidic Cytometer
Microfluidic Systems for Live Cell Imaging
Rise of the micro-machines: microfluidics and the future of cytometry
Label-Free Resistive-Pulse Cytometry
Imaging cytometry
Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry
Analytical capabilities of ImageStream cytometer
Laser scanning cytometry: capturing the immune system in situ
Image cytometry analysis of circulating tumor cells
Pre-clinical Applications of Quantitative Imaging Cytometry to Support Drug Discovery
Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies
Automation of the buccal micronucleus cytome assay using laser scanning cytometry
Laser Scanning Cytometry of Mitosis:state and stage analysis
Instrumentation, new probes and methods
Lasers in Flow Cytometry
The use of hollow fiber membranes combined with cytometry in analysis of bacteriological samples
Guide to red fluorescent proteins and biosensors for flow cytometry
Quantum Dot Technology in Flow Cytometry
Background-free Cytometry Using Rare Earth Complex Bioprobes
Surface Enhanced Raman Scattering (SERS) Cytometry
Recent Advances in Flow Cytometric Cell Sorting
