This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Author(s): Philippe Déjardin
Series: Principles and Practice
Edition: 1
Publisher: Springer
Year: 2006
Language: English
Pages: 341
Contents......Page 7
1.1 Introduction......Page 16
1.2 Theoretical Prediction......Page 17
1.3 Experimental Measure......Page 21
1.4 Results......Page 24
1.5 Discussion......Page 32
References......Page 36
2.1 Introduction......Page 38
2.2 Optical Waveguide Lightmode Spectroscopy......Page 40
2.3 The Practical Determination of Waveguide Parameters......Page 49
2.5 Kinetic Analysis and Dynamic Structural Inference......Page 52
2.6 Behaviour of Real Proteins......Page 58
2.7 Conclusions......Page 62
References......Page 63
3.1 Introduction......Page 65
3.2 The Initial Adsorption Constant and its Limit Expressions......Page 70
3.3 The Structural Transition with Increasing Interfacial Concentration......Page 77
3.4 Conclusion......Page 81
Appendix......Page 82
References......Page 83
4.1 Introduction......Page 88
4.2 Experimental Approaches Adopted......Page 92
4.3 DPI: Applications......Page 93
4.4 Future Developments......Page 104
Appendix 1 DPI: Background......Page 106
Appendix 2 DPI: Theory......Page 108
Appendix 3 DPI: Implementation......Page 112
References......Page 115
5.1 Introduction......Page 118
5.2 Total Internal Reflection Ellipsometry......Page 119
5.3 Experimental Setup......Page 123
5.4 Application Examples......Page 126
5.5 Further Possibilities......Page 130
References......Page 131
6.1 Introduction......Page 132
6.2 Experimental Techniques......Page 138
6.3 Surface Effects on Both Protein Structure and Solvation by the ATR-FTIR Technique......Page 143
References......Page 155
7.1 Introduction......Page 164
7.2 Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)......Page 166
7.3 Analysis of Proteins on Bio-Devices......Page 174
References......Page 182
8.1 Introduction......Page 187
8.2 Gradated Substrate Physicochemistry......Page 189
8.3 Fibronectin Exchange at a Constant Surface Concentration......Page 193
8.4 Fibronectin Exchange at Variable Surface Concentrations......Page 200
8.5 Relevance of the Interfacial Constraints of Fibronectin for Cell-Matrix Adhesion......Page 207
References......Page 209
9.1 Introduction......Page 211
9.2 Nonconducting Cellulosic Textiles......Page 213
9.3 Electron-Conducting Textile......Page 239
References......Page 254
10.1 Introduction......Page 257
10.2 Copolymerization Procedures......Page 258
10.3 Poly(ethylene glycol) Tethering......Page 264
10.4 Physical Adsorption......Page 269
10.5 Biomacromolecule Immobilization......Page 271
10.6 Biomimetic Modification......Page 275
10.7 Conclusion......Page 278
References......Page 280
11.1 Surface Modifications for Reducing Nonspecific Protein Adsorption......Page 282
11.2 Surface-Modified PPMMs for Enzyme Immobilization......Page 297
References......Page 306
12.1 Introduction......Page 310
12.2 Forces Involved in Protein Adsorption......Page 311
12.3 Design of Phosphorylcholine-Bearing Surfaces......Page 313
12.4 Mechanism of Resistance to Protein Adsorption on the MPC Polymer Surface......Page 314
12.5 Fundamental Interactions Between MPC Polymers and Proteins......Page 321
12.6 Recent Designs of Nonfouling Phosphorylcholine Surfaces with Well-Defined Structures......Page 323
12.7 Control of Cell–Material Interactions on a Phosphorylcholine Polymer Nonfouling Surface......Page 325
References......Page 332
D......Page 338
M......Page 339
R......Page 340
Z......Page 341
