This book is a great place to start for anyone wanting to get a good overview of protein purification. The book starts out with an overview of the process and then gets into all the useful details about the conditions and techniques used and is by far one of the best and most importantly one of the thinnest books in the market. There are easy to follow protocols that one could adapt for specific proteins. This is good place to start out and for more detailed information (if required) one could always turn to the cold spring harbor manuals. Recommended for biochemistry graduate students and technicians new to protein purification.
Author(s): Philip L.R. Bonner
Series: THE BASICS Garland Science
Edition: 1
Publisher: Taylor & Francis
Year: 2007
Language: English
Pages: 201
Book Cover......Page 1
Title......Page 4
Copyright......Page 5
Contents......Page 6
Abbreviations......Page 8
Preface......Page 10
1 Protein purification strategy and equipment......Page 12
2 Groundwork......Page 36
3 Nonaffinity absorption techniques for purifying proteins......Page 90
4 Affinity procedures for purifying proteins......Page 108
5 Nonabsorption techniques for purifying proteins......Page 132
6 Monitoring the purity of protein solutions......Page 148
Appendix 1 The common units used in biology......Page 172
Appendix 2 Answers to the exercises......Page 173
Appendix 3 Single-letter code for amino acids......Page 176
Appendix 4 List of suppliers......Page 177
Glossary......Page 186
Index......Page 196
