Leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens.
Author(s): Tuan Vo-Dinh
Series: Methods in Molecular Biology
Edition: 1
Publisher: Humana Press
Year: 2005
Language: English
Pages: 479
Cover......Page 1
Contents......Page 10
1. Protein Nanotechnology......Page 16
2. Kinetics and Mechanisms of Protein Crystallization at the Molecular Level......Page 30
3. Nanostructured Systems for Biological Materials......Page 67
4. Nanomaterials of Drug Delivery Systemsfor Tissue Regeneration......Page 95
5. Nanotechnology With S-Layer Proteins......Page 115
6. Folding of β-Structured Fibrous Proteinsand Self-Assembling Peptides......Page 139
7. Application of NMR Methodsto Identify Detection Reagents for Usein Development of Robust Nanosensors......Page 155
8. Studying 3D Subdomains of Proteinsat the Nanometer Scale Using Fluorescence Spectroscopy......Page 179
9. Carbon Nanotubes and Nanowires for Biological Sensing......Page 205
10. Carbon Nanotube Systems to Communicate With Enzymes......Page 239
11. Molecularly Imprinted Polymersfor Biomolecular Recognition......Page 257
12. Plasmonics-Based Nanostructuresfor Surface-Enhanced Raman Scattering Bioanalysis......Page 269
13. Bacterial Virus φ29 DNA-Packaging Motorand Its Potential Applications in Gene Therapyand Nanotechnology......Page 299
14. Construction of Ordered Protein Arrays......Page 339
15. Bioengineering and Characterizationof DNA–Protein Assemblies Floatingon Supported Membranes......Page 363
16. Nanosystems for Biosensing......Page 383
17. Optical Nanosensors for Detecting Proteinsand Biomarkers in Individual Living Cells......Page 397
18. Nanoelectrodes Integratedin Atomic Force Microscopy Cantileversfor Imaging of In Situ Enzyme Activity......Page 417
19. Protein Amyloidose Misfolding......Page 431
20. Near-Field Scanning Optical Microscopyfor Bioanalysis at Nanometer Resolution......Page 451
Index......Page 467
