Paperback. This book is about liquid chromatography as a tool for isolating and purifying proteins and appears as a handbook-wise linear selection of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase. Part B deals with various target protein classes like membrane proteins, recombinant proteins, and glycoproteins. Finally, Part C looks at various miscellaneous, mutual aspects including coupling reactions, buffer solution additives, and software. The book as a whole can be viewed as a systematic survey of what can be utilized and optimized these days but it also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the essence of the whole contribution. It has been considered very important in this compilation to adhere to the philosophy that practical application is ever
Author(s): Michael Kastner (Eds.)
Series: Journal of Chromatography Library 61
Publisher: Elsevier Science
Year: 1999
Language: English
Commentary: +OCR
Pages: iii-xi, 3-936
Content:
Edited by
Page iii
Copyright page
Page iv
Preface
Pages v-vi
Michael Kastiwr
List of Contributors
Pages vii-x
Overview of Chapterers
Page xi
Chapter 1 Ion Exchange Chromatography Original Research Article
Pages 3-88
Peter H. Roos
Chapter 2 Size Exclusion Chromatography Original Research Article
Pages 89-145
Matthias Rögner
Chapter 3 Reversed-Phase Chromatography Original Research Article
Pages 147-234
Hartmut SchlüTer
Chapter 4 Hydrophobic Interaction Chromatography Original Research Article
Pages 235-269
Lothar R. Jacob
Chapter 5 Hydroxyapatite Chromatography Original Research Article
Pages 271-299
Wolfgang R. Deppert, Richard Lukaĉin
Chapter 6 Immobilized Metal Ion Afinity Chromatography Original Research Article
Pages 301-383
Michael Kastner
Chapter 7 Chromatofocusing Original Research Article
Pages 385-414
Richard Lukaĉ, Wolfgang R. Deppert
Chapter 8 Dye-Ligand Affinity Chromatography Original Research Article
Pages 415-448
Jürgen Kirchberger, Hans-Joachim Böhme
Chapter 9 Immobilized Artificial Membrane Chromatography Original Research Article
Pages 449-468
Kimberly Hauer, Hanlan Liu, Charles Pidgeon
Chapter 10 Liquid-Liquid Partition Chromatography Original Research Article
Pages 469-504
Ulla-Britt Hansson, Christer Wingren
Chapter 11 Displacement Chromatography Original Research Article
Pages 505-524
Hartmut Schlüter, Joachim Jankowski
Chapter 12 Integral Membrane Proteins Original Research Article
Pages 527-556
Gjalt W. Welling, Sytske Welling-Wester
Chapter 13 Recombinant Proteins Original Research Article
Pages 557-586
Otmar Huber, Martina Huber-Wunderlich
Chapter 14 DNA-Binding Proteins Original Research Article
Pages 587-617
Mathias Velleman
Chapter 15 Lectins and Glycoconjugates Original Research Article
Pages 619-638
Hans-Joachim Gabius
Chapter 16 Covalent Chromatography Original Research Article
Pages 639-665
Donald Whitney
Chapter 17 Cell Afinity Chromatography Original Research Article
Pages 667-702
David D. Putnam, Barry F. Wolf, Mark A. Burns
Chapter 18 Coupling Reactions Original Research Article
Pages 705-716
Egbert Müller
Chapter 19 Immobilization of Nucleotides Original Research Article
Pages 717-729
Egbert Müller, Michael Morr
Chapter 20 Miscellaneous Biospecific Affinity Gels Original Research Article
Pages 731-764
Alan M. Williams
Chapter 21 Scale-up of Downstream Processing Original Research Article
Pages 765-783
Joachim Walter
Chapter 22 Chromatography on Porous Matrices Original Research Article
Pages 785-824
Jochen Kruip
Chapter 23 Chromatography Using Strep-tag Affinity Peptides Original Research Article
Pages 825-837
Holger N. Müler, Thomas G.M. Schmidt
Chapter 24 Buffers and Additives Original Research Article
Pages 839-862
Wolfgang R. Deppert, Richard Lukaĉin
Chapter 25 Chromatography Software Original Research Article
Pages 863-876
Thomas Steger-Hartmann
Abbreviations used in this book
Pages 879-885
Directory of Manufacturers and Suppliers
Pages 887-894
Subject Index
Pages 895-936
