With its focus on process development and large-scale bioseparation tasks, this is tailor-made reading for the professional bioengineer in both the biotech and pharmaceutical industries. Following a tried-and-tested concept, this guide has been developed over several years in training courses for biotech and chemical engineers in Europe and the U.S. The first part deals with the theory, introducing chromatography and its dynamics, as well as discussing mass transfer and dispersion effects. The second part then goes on to cover equipment and protocols, determining the retention factor and HETP from isocratic and elution experiments, as well as the mass transfer and intraparticle diffusivity from batch and shallow-bed adsorption experiments.
Author(s): Giorgio Carta, Alois Jungbauer
Edition: 1
Publisher: Wiley-VCH
Year: 2010
Language: English
Pages: 364
Tags: Биологические дисциплины;Биохимия;Методы исследований в биохимии;
Cover......Page 1
Frontmatter......Page 2
Related Titles......Page 3
ISBN: 978-3-527-31819-3......Page 5
Contents......Page 6
Preface......Page 10
Nomenclature......Page 14
1.1 Introduction......Page 19
1.2 Bioproducts and their Contaminants......Page 20
1.3 Bioprocesses......Page 55
1.4 Role of Chromatography in Downstream Processing......Page 67
2.2 Basic Principles and Defi nitions......Page 75
2.3 Modes of Operation......Page 79
2.4 Performance Factors......Page 87
2.5 Separation Performance Metrics......Page 92
3.1 Introduction......Page 103
3.2 Interaction Types and Chemistry......Page 104
3.3 Buffers and Mobile Phases......Page 123
3.4 Physical Structure and Properties......Page 126
4.1 Introduction......Page 143
4.2 Laboratory - scale Systems......Page 144
4.3 Process Columns and Equipment......Page 153
5.1 Introduction......Page 163
5.2 Single Component Systems......Page 165
5.3 Multi - component Systems......Page 175
6.2 Rate Mechanisms......Page 179
6.3 Batch Adsorption Kinetics......Page 197
7.2 Conservation Equations......Page 219
7.3 Local Equilibrium Dynamics......Page 223
7.4 Multi - component Systems......Page 235
7.5 Displacement Development......Page 245
8.1 Introduction......Page 255
8.2 Empirical Characterization of Column Effi ciency......Page 256
8.3 Modeling and Prediction of Column Effi ciency......Page 264
9.1 Introduction......Page 295
9.2 General Theory for Gradient Elution with Linear Isotherms......Page 297
9.3 LGE Relationships for Ion Exchange Chromatography......Page 304
9.4 LGE Relationships for RPC and HIC......Page 313
9.5 Separations with p H Gradients......Page 317
9.6 Modeling Gradient Elution with Non - linear Isotherms......Page 322
10.1 Introduction......Page 327
10.2 Chromatographic Process Steps and Constraints......Page 329
10.3 Design for Capture......Page 331
10.4 Design for Chromatographic Resolution......Page 339
10.5 SMB Design......Page 345
Index......Page 359
