This new and expanded edition of the bestselling textbook has now been fully revised to include techniques from across the biosciences. It focuses particularly on those techniques that have led to recent advances in molecular biology and is designed to help students understand the theory and practical principles behind those methods. The book will be essential reading for all bioscience undergraduate students and pre-clinical medical students and will complement traditional undergraduate texts in biochemistry and molecular biology
Preface; List of contributors; List of abbreviations; 1. Basic priniciples Keith Wilson and John Fyffe; 2. Cell culture techniques Anwar R. Baydoun; 3. Centrifugation Kay Ohlendieck; 4. Microscopy Stephen W. Paddock; 5. Molecular biology, bioinformatics and basic techniques Ralph Rapley; 6. Recombinant DNA and genetic analysis Ralph Rapley; 7. Immunochemical techniques Robin Thorpe and Susan Thorpe; 8. Protein structure, purification, characterisation and functional analysis John Walker; 9. Mass spectrometric techniques Alistair Aitken; 10. Electrophoretic techniques John Walker; 11. Chromatographic techniques Keith Wilson; 12. Spectroscopic techniques I: atomic and molecular electronic spectroscopy Derek B. Gordon; 13. Spectroscopic techniques II: vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields; 14. Radioisotope techniques Robert J. Slater; 15. Enzymes Keith Wilson; 16. Cell membrane receptors and cell signalling Keith Wilson
