In recent years, the role of cilia in the study of health, development and disease has been increasingly clear and new discoveries have made this an exciting and important field of research. This comprehensive volume, a complement to the new three-volume treatment of cilia and flagella by King and Pazour, presents easy-to-follow protocols and detailed background information for researchers working with cilia and flagella.*Covers protocols for primary cilia across several systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians and scientists working in in a wide range of fields
Author(s): Roger D. Sloboda (Eds.)
Series: Methods in Cell Biology 94
Edition: 1
Publisher: Academic Press
Year: 2009
Language: English
Pages: 1-386
Tags: Биологические дисциплины;Цитология;
Content:
Series Editors
Page ii
Volume Editors
Page iii
Copyright
Page iv
Contributors
Pages xi-xiii
Preface
Pages xv-xvi
Chapter 1 - From Central to Rudimentary to Primary: The History of an Underappreciated Organelle Whose Time Has Come.The Primary Cilium
Pages 2-52
Robert A. Bloodgood
Chapter 2 - Origin of the Cilium: Novel Approaches to Examine a Centriolar Evolution Hypothesis
Pages 53-64
Mark C. Alliegro, Peter Satir
Chapter 3 - Using quantitative PCR to Identify Kinesin-3 Genes that are Upregulated During Growth Arrest in Mouse NIH3T3 Cells
Pages 66-86
Rikke I. Thorsteinsson, Søren T. Christensen, Lotte B. Pedersen
Chapter 4 - Methods for the Isolation of Sensory and Primary Cilia: An Overview
Pages 87-101
Kimberly A.P. Mitchell, Gabor Szabo, Angela de S. Otero
Chapter 5 - Isolation of Primary Cilia for Morphological Analysis
Pages 103-115
Bing Huang, Tatyana Masyuk, Nicholas LaRusso
Chapter 6 - Analyzing Primary Cilia by Multiphoton Microscopy
Pages 117-135
Cornelia E. Farnum, Rebecca M. Williams, Eve Donnelly
Chapter 7 - Primary Cilia and the Cell Cycle
Pages 137-160
Olga V. Plotnikova, Elena N. Pugacheva, Erica A. Golemis
Chapter 8 - Utilization of Conditional Alleles to Study the Role of the Primary Cilium in Obesity
Pages 162-179
Robert A. Kesterson, Nicolas F. Berbari, Raymond C. Pasek, Bradley K. Yoder
Chapter 9 - Using Nucleofection of siRNA Constructs for Knockdown of Primary Cilia in P19.CL6 Cancer Stem Cell Differentiation into Cardiomyocytes
Pages 181-197
Christian A. Clement, Lars A. Larsen, Søren T. Christensen
Chapter 10 - The Primary Cilium as a Hedgehog Signal Transduction Machine
Pages 199-222
Sarah C. Goetz, Polloneal J.R. Ocbina, Kathryn V. Anderson
Chapter 11 - Detecting the Surface Localization and Cytoplasmic Cleavage of Membrane-Bound Proteins
Pages 223-239
Hannah C. Chapin, Vanathy Rajendran, Anna Capasso, Michael J. Caplan
Chapter 12 - Assay for In Vitro Budding of Ciliary-Targeted Rhodopsin Transport Carriers
Pages 241-257
Dusanka Deretic, Jana Mazelova
Chapter 13 - Immunoelectron Microscopy of Vesicle Transport to the Primary Cilium of Photoreceptor Cells
Pages 259-272
Tina Sedmak, Elisabeth Sehn, Uwe Wolfrum
Chapter 14 - Polycystic Kidney Disease, Cilia, and Planar Polarity
Pages 273-297
Luis F. Menezes, Gregory G. Germino
Chapter 15 - Constructing and Deconstructing Roles for the Primary Cilium in Tissue Architecture and Cancer
Pages 299-313
E. Scott Seeley, Maxence V. Nachury
Chapter 16 - Polyglutamylation and the fleer Gene
Pages 316-332
Narendra H. Pathak, Iain A. Drummond
Chapter 17 - Regulation of Cilia assembly, Disassembly, and Length by Protein Phosphorylation
Pages 333-346
Muqing Cao, Guihua Li, Junmin Pan
Chapter 18 - Posttranslational Protein Modifications in Cilia and Flagella
Pages 347-363
Roger D. Sloboda
Index
Pages 365-378
Volume in Series
Pages 379-386
Color Plates
Pages C1-C20
