The concept of using photochemical probes in the study of biological systems was developed by Westheimer who published the first photoaffinity labeling experiments more than twenty years ago (J.Bio1. Chem. 237, (1962) 3006). Since then the concept has been used successfully in various areas of biochemistry and recently several new interesting and exciting aspects of the concept have been developed. It is the general opinion by scientists in the "field" that the full potential of photochemical probes in biochemical studies has far from been exploited yet. This is mostly due to the interdisciplinary character of the concept involving photochemistry, synthetic chemistry as well as biochemistry/molecular biology. The perspective of the NATO advanced workshop on "Photochemical Probes in Biochemistry", held in Holte (Copenhagen) Denmark 14-19, August, 1988, was several fold. The workshop was to give an account of the "state of the art" of using photochemical probes in biochemistry as well as to bring together specialists in photochemistry, syn thetic chemistry and molecular biology in order to analyze advantages as well as the inherent problems and pitfalls of the concept and provide suggestions and guidelines for fu ture research. Furthermore, it is the hope of the editor that the present publication which gives an account of the lectures presented at the workshop, will provide an introduction to scientists who are not familiar with photochemical probes, but to whom these could help answer central and pertinent questions.
Author(s): Ole Buchardt, Ulla Henriksen, Troels Koch (auth.), Dr. Peter E. Nielsen (eds.)
Series: NATO ASI Series 272
Edition: 1
Publisher: Springer Netherlands
Year: 1989
Language: English
Pages: 320
Tags: Biochemistry, general; Organic Chemistry
Front Matter....Pages i-ix
Photolabeling Reagent Design....Pages 1-9
Nucleophilic Aromatic Photo Substitutions on Nitrophenyl Ethers. A New Photoaffinity Labelling Technique?....Pages 11-29
Photochemistry of Aryl and Aroyl Azides: Application to Photolabeling of Biological Systems....Pages 31-41
Membrane Protein Labelling with Photoreactive Phospholipid Analogues....Pages 43-58
125 J-ASA-PE, A Photoactivable, Radioactive Phospholipid-Analogue, Designed for Photolabeling Proteins in Contact with Phospholipid Head Groups....Pages 59-65
Photoaffinity Labelling of Receptors for Melanocyte-Stimulating Hormone....Pages 67-84
Topographical Analysis of the Torpedo Marmorata Acetylcholine Receptor by Energy Transfer Photoaffinity Labeling Using Aryldiazonium Derivatives....Pages 85-105
Photosuicide Labelling....Pages 107-122
Antibiotic Photoaffinity Labeling Probes of Escherichia Coli Ribosomal Structure and Function....Pages 123-139
Dissection of the Ribosomal Peptidyl Transferase Center by Photoaffinity Labeling: P- And A-Sites Are Located At Different Positions in Domain V of 23 S RNA....Pages 141-156
Studying the Cytoskeleton by Label Transfer Crosslinking: Uses and Limitations....Pages 157-168
RecA-Directed Hybridization of Psoralen-Monoadducted DNA Oligonucleotides to Duplex Targets....Pages 169-177
The use of Psoralen-Photocrosslinking for the Analysis of the Chromatin Structure during Transcription....Pages 179-194
Metal Complexes as Photochemical Probes of DNA Structure....Pages 195-197
Laser-Induced Strand Break Formation of Polyuridylic Acid in the Presence and Absence of Tris(2,2’-Bipyridyl)Ruthenium Chloride and K 2 S 2 O 8 ....Pages 199-208
Photonucleases....Pages 209-218
Sequence-Targeted Photochemical Reactions in Single-Stranded and Double-Stranded Nucleic Acids by Oligonucleotide-Photosensitizer Conjugates....Pages 219-229
Photofootprinting Analysis of Protein-DNA Interactions....Pages 231-240
Studies of the Binding and Biological Actions of Ethidium....Pages 241-259
Quantitative photoaffinity labeling of Escherichia coli RNA polymerase transcription complexes by nascent RNA....Pages 261-275
‘CAGED’ Compounds to Probe the Dynamics of Cellular Processes: Synthesis and Properties of some Novel Photosensitive P-2-Nitrobenzyl Esters of Nucleotides....Pages 277-296
Back Matter....Pages 297-308
