The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis.
Author(s): Susan M. Lunte and Donna M. Radzik (Eds.)
Series: Progress in Pharmaceutical and Biomedical Analysis 2
Edition: 1st
Publisher: Elsevier, Academic Press
Year: 1996
Language: English
Pages: 3-511
Content:
List of contributors
Pages vii-viii
Introduction
Pages ix-x
Susan M. Lunte, Donna M. Radzik
Chapter 1 Principles of capillary electrophoresis Original Research Article
Pages 3-21
Susan M. Lunte, Donna M. Radzik
Chapter 2 Column technologies for capillary electrophoresis Original Research Article
Pages 23-80
John K. Towns, Fred E. Regnier
Chapter 3 Electrokinetic chromatography Original Research Article
Pages 81-178
Joseph P. Foley, Eric S. Ahuja
Chapter 4 Optical detection strategies in capillary electrophoresis Original Research Article
Pages 181-228
Steven A. Soper
Chapter 5 Capillary electrophoresis/Mass spectrometry Original Research Article
Pages 229-275
Richard D. Smith, Harold R. Udseth
Chapter 6 Electrochemical detection for capillary electrophoresis Original Research Article
Pages 277-306
Thomas J. O'shea
Chapter 7 Sample preparation for capillary electrophoresis Original Research Article
Pages 309-326
David K. Lloyd
Chapter 8 Capillary electrophoresis in the study of amino acids Original Research Article
Pages 327-344
Paul L. Weber
Chapter 9 Capillary electrophoresis of proteins and peptides Original Research Article
Pages 345-386
Kathleen L. Kostel
Chapter 10 Separation of nucleic acids and oligonucleotides and sequencing of DNA Fragments by capillary electrophoresis Original Research Article
Pages 387-424
Ram P. Singhal, Jun Xian
Chapter 11 The Use of Capillary Electrophoresis in Pharmaceutical Development Original Research Article
Pages 425-466
Sou Chan Chang, John J. Gigantino, Donna M. Radzik, Allen C. Rychtman
Chapter 12 Capillary electrophoresis-based methods for in vivo analysis: Single cell analysis and microdialysis sampling Original Research Article
Pages 467-502
Barry L. Hogan
Index
Pages 503-511
