The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited (by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) Complete retrieval of novel expressed genes by PCR without screening a library Quantitation by PCR Mutagenesis by PCR PCR in AIDS research Simple and effective protocols for PCR on archival specimens
Author(s): Gobinda Sarkar (Eds.)
Series: Methods in Neurosciences 26
Publisher: Academic Press
Year: 1995
Language: English
Pages: 3-455
Content:
Contributors to volume 26
Pages ix-xiv
Preface
Page xv
Gobinda Sarkar
Methods in Neurosciences
Pages xvii-xviii
[1] Formamide low-temperature PCR: Applications for direct PCR from clinical material Original Research Article
Pages 3-14
Michael Panaccio, Michelle French, Andrew Lew
[2] Direct use of blood in PCR Original Research Article
Pages 15-25
Bjørn-Yngvar Nordvåg, Hilde Monica F. Riise, Gunnar Husby, Inge Nilsen, M. Raafat El-Gewely
[3] Use of PCR for isolation of neuropeptide receptor genes Original Research Article
Pages 29-44
Tapan K. Chatterjee, Gobinda Sarkar, Rory A. Fisher, Mark E. Bolander
[4] Lock-docking rapid amplification of cDNA ends PCR: Strategies and applications Original Research Article
Pages 45-61
Nancy D. Borson, Wilmar L. Salo, Lester R. Drewes
[5] Single-cell cDNA-PCR Original Research Article
Pages 62-74
Benedikt L. Ziegler, Christa P. Lamping, Stefan J. Thoma, Theodor M. Fliedner, Christian A. Thomas
[6] PCR-mediated synthesis of chimeric molecules Original Research Article
Pages 77-85
André Darveau, Alex Pelletier, Josée Perreault
[7] PCR-mediated gene synthesis Original Research Article
Pages 86-102
Krishna Jayaraman
[8] Quantitative PCR: Analysis of rare mitochondrial DNA mutations in central nervous system tissues Original Research Article
Pages 105-128
Nay Wei Soong, Norman Arnheim
[9] PCR-aided transcript titration assay: Competitive PCR for evaluation of absolute levels of rare mRNA species Original Research Article
Pages 129-146
Michael Becker-André
[10] Comparative evaluation of quantitative PCR methods Original Research Article
Pages 147-160
Sanjiv Sur, Gerald J. Gleich, Mark E. Bolander, Gobinda Sarkar
[11] Mutational detection by single-strand conformational polymorphism Original Research Article
Pages 163-183
Nabeel Bardeesy, Jerry Pelletier
[12] Detection of mutation in yeast hsp60 gene by PCR Original Research Article
Pages 184-193
A. Sanyal, G.S. Getz
[13] Comparison of the sensitivity of single-strand conformational polymorphism and heteroduplex methods Original Research Article
Pages 194-209
Damjan Glavač, Michael Dean
[14] Analysis of p53 mutations in human gliomas by RNA single-strand conformational polymorphism Original Research Article
Pages 210-224
Theresa M. Cheng, Vinod Ganju, Steve R. Ritland, Gobinda Sarkar, Robert B. Jenkins
[15] PCR and generation of antisense RNA probes for use in RNase protection assays Original Research Article
Pages 227-242
Honghao Yang, Peter W. Melera
[16] In vitro transcription of cRNA from PCR-generated DNA fragments Original Research Article
Pages 243-251
Sherry Leonard
[17] Direct cloning of DNA fragments generated by PCR Original Research Article
Pages 255-261
David Kovalic, Bernard Weisblum
[18] Subtractive cDNA cloning using oligo(dT)30-latex and PCR Original Research Article
Pages 262-276
E. Hara, K. Oda, Y. Furuichi
[19] Expression cloning: PCR versus episomal vectors for rescue of transfected genes Original Research Article
Pages 277-289
Kenton S. Miller, Mark Brudnak
[20] Use of PCR for constructing jumping libraries Original Research Article
Pages 290-306
Rajendra P. Kandpal, Sherman M. Weissman
[21] Site-directed mutagenesis by PCR: Substitution, insertion, deletion, and gene fusion Original Research Article
Pages 309-323
Sailen Barik
[22] Use of PCR in analysis of 5′-flanking region of androgen receptor gene Original Research Article
Pages 324-336
M. Vijay Kumar, Donald J. Tindall
[23] Detection of HIV-1 in brain tissue of individuals with AIDS by in situ gene amplification Original Research Article
Pages 339-357
Omar Bagasra, Roger J. Pomerantz
[24] Estimation of genetic heterogeneity in primate T-cell lymphoma/leukemia viruses by PCR Original Research Article
Pages 358-380
D.K. Dube, S. Dube, M.P. Sherman, J. Love, N.K. Saksena, B.J. Poiesz, W.J. Harrington Jr., J.F. Ferrer, L. Papsidero, L. Dyster, R. Yanagihara, A.E. Williams, J.B. Glaser, V.M.A. Herve, F. Barre-Sinoussi, B.S. Blumberg
[25] Use of PCR in detection of antisense transcripts in HTLV-I-infected patients and human T-cell lines Original Research Article
Pages 381-400
S. Dube, B. Paul, V. Bryz-Gornia, C. Stephens, S. Erensoy, B.J. Poiesz, D.K. Dube
[26] Direct chemiluminescent sequencing of double-stranded PCR products Original Research Article
Pages 403-413
Andrea M. Douglas, Bentley A. Atchison
[27] Use of PCR to determine genomic DNA target sites for zinc finger protein expressed in mouse cerebellum Original Research Article
Pages 414-430
Annette Christoph, Hans-Jürgen Thiesen
[28] DNA extraction from archived specimens by sonication Original Research Article
Pages 431-443
Robert A. Robinson, Michael J. Heller
Index
Pages 445-455
