In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
Author(s): Franco Quercioli (auth.), Alberto Diaspro (eds.)
Edition: 1
Publisher: Springer-Verlag Berlin Heidelberg
Year: 2011
Language: English
Pages: 244
Tags: Biological Microscopy;Cell Biology;Biophysics and Biological Physics;Biochemistry, general
Front Matter....Pages i-xii
Fundamentals of Optical Microscopy....Pages 1-36
The White Confocal: Continuous Spectral Tuning in Excitation and Emission....Pages 37-54
Second/Third Harmonic Generation Microscopy....Pages 55-74
Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles....Pages 75-84
New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy....Pages 85-99
Improving Image Formation by Pushing the Signal-to-Noise Ratio....Pages 101-110
Site-Specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes....Pages 111-130
Imaging Molecular Physiology in Cells Using FRET-Based Fluorescent Nanosensors....Pages 131-152
Measuring Molecular Dynamics by FRAP, FCS, and SPT....Pages 153-163
In Vitro–In Vivo Fluctuation Spectroscopies....Pages 165-181
Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking....Pages 183-189
Low Concentration Protein Detection Using Novel SERS Devices....Pages 191-210
Near Infrared Three-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation....Pages 211-229
A Correlative Microscopy: A Combination of Light and Electron Microscopy....Pages 231-238
Back Matter....Pages 239-244
