Opsin-free Optogenetics: Technology and Applications

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Optogenetics represents a breakthrough technology capable of dynamically modulating molecular and cellular activity in live cells with high precision. This transformative technology made it possible to reversibly interrogate protein actions, cellular events, and animal behaviors with a simple flash of light. Manipulating the temporal and spatial profile of light enabled precise control of membrane potentials. This feature has inspired non-opsin-based optogenetics, which not only inherits the precise spatiotemporal resolution but also expands the targets into a diverse pool of biomolecules such as membrane receptors, ion channels, kinases, GTPases, and transcription factors. This book is unique because it emphasizes the design and applications of opsin-free optogenetic tools.

Key Features

    • Describes new developments in non-opsin-based optogenetic tools

    • Introduces cutting-edge methods for precise modulation of cell signaling

    • Highlights applications of optogenetic regulation of cellular functions

    • Covers contributions from an international team of leading experts

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      Author(s): Yubin Zhou, Kai Zhang
      Series: Methods in Signal Transduction Series
      Publisher: CRC Press
      Year: 2022

      Language: English
      Pages: 201
      City: Boca Raton

      Cover
      Half Title
      Series
      Title
      Copyright
      Content
      Preface
      Acknowledgments
      Editor Bios
      List of Contributors
      Chapter 1 Optogenetic Dissection of a Two-Component Calcium Influx Pathway
      Chapter 2 High-Throughput Engineering of a Light-Activatable Ca2+ Channel
      Chapter 3 Optogenetic Activation of TrkB Signaling
      Chapter 4 Spatiotemporal Modulation of Neural Repair
      Chapter 5 Optogenetic Control of Neural Stem Cell Differentiation
      Chapter 6 An Optogenetic Toolbox for Remote Control of Programmed Cell Death
      Chapter 7 Control of Protein Levels in Saccharomyces cerevisiae by Optogenetic Modules that Act on Protein Synthesis and Stability
      Chapter 8 Optogenetics as a Tool to Study Neurodegeneration and Signal Transduction
      Chapter 9 Opsin-free Optogenetics: Brain and Beyond
      Chapter 10 Constructing a Far-Red Light-Induced Split-Cre Recombinase System for Controllable Genome Engineering
      Chapter 11 Tools and Technologies for Wireless and Non-Invasive Optogenetics
      Index