This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Author(s): Christine E. Engeland
Series: Methods in Molecular Biology
Publisher: Springer Science+Business Media, LLC, part of Springer Nature 2020
Year: 2020
Language: English
Pages: 332
Front Matter ....Pages i-xi
Introduction to Oncolytic Virotherapy (Christine E. Engeland, John C. Bell)....Pages 1-6
Methods for Modification of Therapeutic Viruses (Claudia A. P. Hill, Luca Bau, Robert Carlisle)....Pages 7-29
Tumor Targeting of Oncolytic Adenoviruses Using Bispecific Adapter Proteins (Julia Niemann, Florian Kühnel)....Pages 31-49
Development of Entry-Targeted Oncolytic Measles Viruses (Michael D. Mühlebach, Roberto Cattaneo)....Pages 51-75
Insert Stability and In Vivo Testing of MicroRNA-Detargeted Oncolytic Picornaviruses (Autumn J. Schulze)....Pages 77-94
Ex Vivo Virotherapy with Myxoma Virus to Treat Cancer (Nancy Y. Villa, Lina S. Franco, Grant McFadden)....Pages 95-110
Immunomodulation in Oncolytic Measles Virotherapy (Laura Dietz, Christine E. Engeland)....Pages 111-126
A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death (Tiphaine Delaunay, Carole Achard, Marc Grégoire, Frédéric Tangy, Nicolas Boisgerault, Jean-François Fonteneau)....Pages 127-132
Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation (Gayathri Vijayakumar, Dmitriy Zamarin)....Pages 133-154
Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue (Krishna Das, Carles Urbiola, Bart Spiesschaert, Philipp Mueller, Guido Wollmann)....Pages 155-177
Immunohistochemistry for Tumor-Infiltrating Immune Cells After Oncolytic Virotherapy (Dipongkor Saha, Samuel D. Rabkin)....Pages 179-190
Detection of Tumor Antigen-Specific T-Cell Responses After Oncolytic Vaccination (Jonathan G. Pol, Byram W. Bridle, Brian D. Lichty)....Pages 191-211
Evaluation of Oncolytic Virus-Induced Therapeutic Tumor Vaccination Effects in Murine Tumor Models (Rūta Veinalde)....Pages 213-227
Delivery of Oncolytic Reovirus by Cell Carriers (Elizabeth J. Ilett)....Pages 229-236
In Vivo Bioimaging for Monitoring Intratumoral Virus Activity (Liesa-Marie Schreiber, Carles Urbiola, Patrik Erlmann, Guido Wollmann)....Pages 237-248
Oncolytic Immunotherapy for Bladder Cancer Using Coxsackie A21 Virus: Using a Bladder Tumor Precision-Cut Slice Model System to Assess Viral Efficacy (Kate Relph, Nicola Annels, Chris Smith, Marcos Kostalas, Hardev Pandha)....Pages 249-259
Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity (Eleanor M. Scott, Sally Frost, Hena Khalique, Joshua D. Freedman, Len W. Seymour, Janet Lei-Rossmann)....Pages 261-270
Generating Primary Models of Human Cancer to Aid in the Development of Clinically Relevant Oncolytic Viruses (Brian A. Keller, Marie-Ève Wedge, Abera Surendran, Carolina S. Ilkow)....Pages 271-284
Considerations for Clinical Translation of MG1 Maraba Virus (Caroline J. Breitbach)....Pages 285-293
Fluorescence In Situ Hybridization (FISH) Detection of Viral Nucleic Acids in Oncolytic H-1 Parvovirus-Treated Human Brain Tumors (Irina Kiprianova, Alexandra Just, Barbara Leuchs, Jean Rommelaere, Assia L. Angelova)....Pages 295-306
Mathematical Modeling of Oncolytic Virotherapy (Johannes P. W. Heidbuechel, Daniel Abate-Daga, Christine E. Engeland, Heiko Enderling)....Pages 307-320
Back Matter ....Pages 321-329
