Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts.
In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
Author(s): Thomas Wartmann, Gotthard Kunze (auth.), Prof. Dr. Klaus Wolf, Prof. Dr. Karin Breunig, Prof. Dr. Gerold Barth (eds.)
Series: Springer Lab Manuals
Edition: 1
Publisher: Springer-Verlag Berlin Heidelberg
Year: 2003
Language: English
Pages: 494
Tags: Eukaryotic Microbiology; Applied Microbiology; Animal Biochemistry; Microbial Genetics and Genomics
Front Matter....Pages I-XXVI
Front Matter....Pages 1-1
Temperature-Dependent Dimorphism of the Non-Conventional Yeast Arxula adeninivorans ....Pages 3-6
Expression of Heterologous Genes in Arxula adeninivorans Budding Cells and Mycelia....Pages 7-12
Front Matter....Pages 13-13
Effect of Precursor Feeding on Riboflavin Production of Ashbya gossypii and Influence of Threonine Aldolase Overexpression....Pages 15-20
Front Matter....Pages 21-21
Characterization of the 26S-rRNA Gene to Classify an Industrial Strain to be Candida maltosa ....Pages 23-28
Isolation of a GCN4 Gene Analog and Determination of Its Involvement in Induction of Cycloheximide-Resistant Ribosomal Protein L41-Q in Candida maltosa ....Pages 29-34
Dehalogenation During Biodegradation of Chlorophenols by Candida maltosa ....Pages 35-38
Production of Aromatic Acids During the Oxidation of Phenylalkanes by Candida maltosa ....Pages 39-44
Front Matter....Pages 45-45
Growth of Debaryomyces hansenii in Seawater Culture Medium....Pages 47-49
Glycerol and Glucose Dissimilation by Debaryomyces hansenii: Substrate Influence on Growth Rate and Biomass Yield of Yeast....Pages 51-57
Induction of GPD from the Halotolerant Yeast Debaryomyces hansenii by Salt Stress....Pages 59-63
Salt Tolerance in Debaryomyces hansenii . Effect of Sodium on Growth at High Temperature and at Extreme pH....Pages 65-68
Preparation of Total DNA from Debaryomyces hansenii ....Pages 69-73
Front Matter....Pages 75-75
Life Cycle of Hansenula polymorpha ....Pages 77-81
Obtention of Auxotrophic Mutants in Hansenula polymorpha ....Pages 83-88
Transformation by Electroporation of the Yeast Hansenula polymorpha ....Pages 89-96
Expression of Hansenula polymorpha Nitrate Reductase Under the Control of the M0X1 Promoter....Pages 97-100
Promoter Assessment in Hansenula polymorpha Using a lacZ Reporter Gene....Pages 101-115
Immuno-Gold Labeling of Invertase in Hansenula polymorpha ....Pages 117-123
Front Matter....Pages 125-125
Determination of Intra- and Extracellular Concentration of Glycerol in the Halophilic Black Yeast Hortaea werneckii Grown at Different Environmental Salinities....Pages 127-130
Regulation of HMGCoA Reducíase Activity by Salt Stress in Hortaea werneckii ....Pages 131-134
Front Matter....Pages 125-125
Isolation and Identification of Lipids from the Halophilic Black Yeast Hortaea werneckii ....Pages 135-142
Inhibition of DHN-Melanin Biosynthesis by Tricyclazole in Hortaea Werneckii ....Pages 143-148
Front Matter....Pages 149-149
Mating, Sporulation and Tetrad Analysis in Kluyveromyces lactis ....Pages 151-154
Isolation of Mitochondrial Mutants from the Petite-Negative Yeast Kluyveromyces lactis ....Pages 155-159
Gene Disruption in the Yeast Kluyveromyces lactis ....Pages 161-167
Transformation of Kluyveromyces lactis ....Pages 169-174
Removal of Dominant Markers from the Kluyveromyces lactis Genome Using the Cre/loxP System....Pages 175-178
Phenotypic Analysis of the Kluyveromyces lactis Killer Phenomenon....Pages 179-183
Genetic Manipulation of the Kluyveromyces lactis Killer Plasmids k1 and k2....Pages 185-190
Chitin-Binding Capability of the Zymocin Complex from Kluyveromyces lactis ....Pages 191-194
Heterologous Protein Production in High Copy Number Vector Systems....Pages 195-199
Alcohol Dehydrogenase (ADH) Isozymes in Kluyveromyces lactis: Detection by Activity....Pages 201-207
Kluyveromyces lactis Genome Mapping and Sequencing....Pages 209-211
Front Matter....Pages 213-213
Mating and Segregation in Pichia stipitis ....Pages 215-219
ADH1 Disruption in Pichia stipitis by Targeted Restriction Enzyme Mediated Integration (tREMI)....Pages 221-228
Examination of Xylose Fermentation in Pichia stipitis by Means of the Sensomat System....Pages 229-233
Regulation of Fermentation and Respiration....Pages 235-240
Investigation of Transcriptional Regulation of the Fermentative ADH in Pichia stipitis Using an EGFP Reporter Gene....Pages 241-244
Cyanide-Insensitive Respiration....Pages 245-252
Freeze Transformation, Plasmid Reisolation and Stability in Pichia stipitis ....Pages 253-259
Front Matter....Pages 261-261
Objective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis ....Pages 263-266
Subjective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis ....Pages 267-269
Analysis of Structural Discontinuities of the Wall of Schwanniomyces occidentalis ....Pages 271-274
Induction of Amylases in Schwanniomyces occidentalis ....Pages 275-278
Ethanol Production from Starch by Schwanniomyces occidentalis ....Pages 279-282
Front Matter....Pages 283-283
Characterization of Soluble Cytochrome P450 from Trichosporon domesticum ....Pages 285-290
Front Matter....Pages 291-291
Oxidation of the Fungicide Biphenyl by Yeasts of the Genus Trichosporon ....Pages 293-296
Front Matter....Pages 297-297
Genetic Complementation Analysis by Protoplast Fusion of Xanthophyllomyces dendrorhous ....Pages 299-304
Lethal Effect of UV Light and Photoreactivation in Xanthophyllomyces dendrorhous ....Pages 305-308
Fermentation and Carotenoid Analysis of the Yeast Xanthophyllomyces dendrorhous [Phaffia rhodozyma) ....Pages 309-313
Metabolic Engineering of the Carotenoid Biosynthetic Pathway in Xanthophyllomyces dendrorhous [Phaffia rhodozyma) ....Pages 315-322
Astaxanthin Biosynthesis in Xanthophyllomyces dendrorhous ( Phaffia rhodozyma ): Effect of Inhibitors and Low Temperature....Pages 323-328
Purification and Characterization of Extrachromosomal Genetic Elements of Double-Stranded RNA (dsRNA) of Xanthophyllomyces dendrorhous ....Pages 329-332
Front Matter....Pages 333-333
Mating, Sporulation and Random Spore Selection by Nystatin in Yarrowia lipolytica ....Pages 335-338
Induction of the Yeast-Hypha Transition in Yarrowia lipolytica ....Pages 339-342
Tagging of Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica ....Pages 343-356
Detection and Activation of the Ylt1 Retrotransposon in the Yeast Yarrowia lipolytica ....Pages 357-364
Flow Cytometry of Yarrowia lipolytica ....Pages 365-368
A Copper-Regulated Yarrowia lipolytica Vector for the Expression of Heterologous Proteins....Pages 369-371
Lipase Assay in Yarrowia lipolytica ....Pages 373-377
Front Matter....Pages 333-333
Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media....Pages 379-384
Detection of Biosynthesis and Degradation of Peroxisomes by Different Carbon Sources with a Combined Assay Using a β-Galactosidase-GFP-SKL Chimaeric Protein for Labeling of Peroxisomes and the Peroxisomal Isocitrate Lyase as Control....Pages 385-392
Induction of Citric Acid/lsocitric Acid and α-Ketoglutaric Acid Production in the Yeast Yarrowia lipolytica ....Pages 393-400
Regulation of Production of Yarrowia lipolytica Extracellular Ribonuclease and Alkaline Extracellular Protease....Pages 401-406
Front Matter....Pages 407-407
Zygosaccharomyces bailii: A Yeast With a Peculiar Pattern for the Regulation of Acetic Acid Metabolism in the Presence of Glucose....Pages 409-416
Transfection of Yeast With Toxin-Coding dsRNA Viruses of Zygosaccharomyces bailii and Toxin Purification by Receptor-Mediated Affinity Chromatography....Pages 417-425
Front Matter....Pages 427-427
Heat-Shock-Induced Activation of Trehalase in the Yeast Zygosaccharomyces rouxii ....Pages 429-433
Front Matter....Pages 435-435
Linear and Circular Plasmids of Yeasts....Pages 437-443
Rapid Method to Extract DNA from Capsulated Yeast Species....Pages 445-449
Testing Various Yeasts for Ethanol Production from Xylose....Pages 451-455
Use of a Differential Culture Medium for the Enumeration of Zygosaccharomyces bailii, Saccharomyces cerevisiae and Pichia membranifaciens in Wine....Pages 457-462
Characterization of Ubiquinone Type (CoQ7–CoQ10) of Non-Conventional Yeasts....Pages 463-467
Non-Radioactive Dot Blot DNA Reassociation for Unequivocal Yeast Identification....Pages 469-474
Taxonomy Rules and Classification Hints for Non-Taxonomists Working with Unknown or JII-Identified Yeast Cultures....Pages 475-485
Back Matter....Pages 486-494
