Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field. Key Features * Comprehensive protocols included for * Detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques * Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression * Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone * Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution
Author(s): Mahin D. Maines (Eds.)
Series: Methods in Neurosciences 31
Edition: 1
Publisher: Academic Press
Year: 1996
Language: English
Pages: 1-354
Content:
Contributors
Pages ix-xiii
Preface
Page xv
Mahin D. Maines
Methods in neurosciences
Pages xvii-xviii
[1] Electron paramagnetic resonance detection of nitric oxide in biological samples Original Research Article
Pages 3-13
Lori G. Rochelle, Harriet Kruszyna, Roger P. Smith
[2] Electrochemical and spectroscopic methods of nitric oxide detection Original Research Article
Pages 14-33
Tadeusz Malinski, Eugeniusz Kubaszewski, Frederick Kiechle
[3] Detection of S-Nitrosothiols Original Research Article
Pages 34-40
George N. Welch, Gilbert R. Upchurch Jr., Joseph Loscalzo
[4] Nitrosation of proteins Original Research Article
Pages 41-46
Ying-Yi Zhang, Joseph Loscalzo
[5] Immunohistochemical methods for nitric oxide synthase Original Research Article
Pages 47-61
Paul K. Nakane
[6] NADPH Diaphorase staining Original Research Article
Pages 62-67
Ted M. Dawson, Valina L. Dawson
[7] Immunohistochemical visualization of cyclic nucleotides Original Research Article
Pages 68-79
Jan de Vente, Heather M. Young, Harry W.M. Steinbusch
[8] Detection of downstream components of cyclic GMP cascade by in situ hybridization analysis Original Research Article
Pages 80-111
Michael F. Goy, Zhiping Li, Ellen M. Zimmermann, David R. Repaske
[9] Detection of heme oxygenase-1 and -2 transcripts by northern blot and in Situ hybridization analysis Original Research Article
Pages 112-122
James F. Ewing
[10] Purification of endothelial nitric oxide synthase Original Research Article
Pages 125-129
Jennifer S. Pollock, Masaki Nakane
[11] Purification of brain nitric oxide synthase from baculovirus overexpression system and determination of cofactors Original Research Article
Pages 130-139
Bernd Mayer, Peter Klatt, Christian Harteneck, Barbara M. List, Ernst R. Wierner, Kurt Schmidt
[12] Prokaryotic expression, purification, and characterization of intact and modular constructs of neuronal nitric oxide synthase Original Research Article
Pages 140-151
Bettie Sue Siler Masters, Kirk McMillan, Linda J. Roman, Pavel Martasek, Jonathan Nishimura
[13] Generation of monoclonal antibodies to endothelial nitric oxide synthase Original Research Article
Pages 152-155
Jennifer S. Pollock, Masaki Nakane
[14] Genetic modulation of inducible nitric oxide synthase expression Original Research Article
Pages 156-172
Edith Tzeng, Timothy R. Billiar
[15] Expression of constitutive brain nitric oxide synthase and inducible nitric oxide synthase by cultured neurons and glia Original Research Article
Pages 173-183
Dahlia Minc-Golomb, Joan P. Schwartz
[16] Structural characterization of human neuronal nitric oxide synthase gene: Methodologic approach to a complex transcription unit Original Research Article
Pages 184-196
Anthony H. Cheung, Philip A. Marsden
[17] Transcriptional control of endothelial nitric oxide synthase gene expression Original Research Article
Pages 197-206
William C. Sessa
[18] Site-Directed mutagenesis to probe endothelial nitric oxide synthase posttranslational modifications Original Research Article
Pages 207-212
Jianwei Liu, William C. Sessa
[19] Nitric oxide and endothelial regulation of vascular tone Original Research Article
Pages 215-227
Francesco Cosentino, Thomas F. Lüscher
[20] Function of nitric oxide in neuronal cell death Original Research Article
Pages 228-240
Valina L. Dawson, Ted M. Dawson
[21] Nitric oxide-cyclic guanosine monophosphate pathway in central nervous system: in vitro and in vivo investigations Original Research Article
Pages 241-252
Mark Salter, Eric Southam, John Garthwaite
[22] Nitric oxide in regulation of microvascular tone: Methods of detection Original Research Article
Pages 253-263
Ulrich Pohl
[23] Regulation of cerebral circulation by nitric oxide Original Research Article
Pages 264-272
Frank M. Faraci
[24] Nitric oxide in NAD/NADH-Dependent protein modification Original Research Article
Pages 273-281
Bernhard Brüne, Eduardo G. Lapetina
[25] Assessment of synaptic effects of nitric oxide in hippocampal neurons Original Research Article
Pages 282-299
Charles F. Zorumski, Steven Mennerick, Yukitoshi Izumi
[26] Synaptic transmission in hippocampal slice Original Research Article
Pages 300-308
Erin M. Schuman
[27] Redox congeners of nitric oxide, N-methyl-d-aspartate receptors, and Intracellular calcium ion Original Research Article
Pages 309-318
Won-Ki Kim, Jonathan S. Stamler, Stuart A. Lipton
[28] Cytosolic and nuclear calcium imaging by confocal microscopy Original Research Article
Pages 319-328,I
Michel Burnier, Gabriel Centeno, Hans R. Brunner
[29] Effects of nitrogen monoxide on cellular iron metabolism Original Research Article
Pages 329-345
Des R. Richardson, Prem Ponka
Index
Pages 347-354
