Fluorescence is more and more widely used as a tool of investigation, analysis, control and diagnosis in many fields relevant to physical, chemical, biological and medical sciences. New technologies con tinuously emerge thanks to the progress in the design of light sources (e.g. laser diodes), detectors (3D, 4D) and compact ultrafast elec tronic devices. In particular, much progress has been made in time resolved fluorescence microscopy (FUM: Fluorescence Lifetime Imaging Microscopy; FCS: Fluorescence Correlation Spectroscopy). Furthermore, the sensitivity now allows one to detect a single mole cule in the restricted field of a confocal microscope, which actually offers the possibility to study phenomena at a molecular level. The development of new fluorescent probes is still a necessity. In particular, the growing use of lasers implies high resistance to photo degradation. Fluorescence emission at long wavelengths is also a distinct advantage. Furthermore, in vivo inclusion of new fluorescent aromatic residues in proteins offer new potentialities in biology. of ions and molecules is Fluorescence-based selective detection still the object of special attention. Considerable effort is being made in the design of supramolecular systems in which the recognition event is converted into a fluorescence signal easily detected. New fluorescent sensors for clinical diagnosis and detection of pollutants in atmosphere and water are extensively developed. All these developments justify the regular publication of books giving the state-of-the-art of the methods and applications of fluo rescence spectroscopy.
Author(s): B Valeur (auth.), Professor Bernard Valeur, Dr. Jean-Claude Brochon (eds.)
Series: Springer Series on Fluorescence 1
Edition: 1
Publisher: Springer-Verlag Berlin Heidelberg
Year: 2001
Language: English
Pages: 490
Tags: Analytical Chemistry; Physical Chemistry; Control, Robotics, Mechatronics
Front Matter....Pages I-XXV
Front Matter....Pages 1-1
Introduction: On the Origin of the Terms Fluorescence, Phosphorescence, and Luminescence....Pages 3-6
Pioneering Contributions of Jean and Francis Perrin to Molecular Luminescence....Pages 7-33
The Seminal Contributions of Gregorio Weber to Modern Fluorescence Spectroscopy....Pages 35-58
Front Matter....Pages 59-59
Investigation of Femtosecond Chemical Reactivity by Means of Fluorescence Up-Conversion....Pages 61-80
Spectroscopic Investigations of Intermolecular Interactions in Supercritical Fluids....Pages 81-97
Space and Time Resolved Spectroscopy of Two-Dimensional Molecular Assemblies....Pages 99-124
From Cyanines to Styryl Bases — Photophysical Properties, Photochemical Mechanisms, and Cation Sensing Abilities of Charged and Neutral Polymethinic Dyes....Pages 125-155
Phototunable Metal Cation Binding Ability of Some Fluorescent Macrocyclic Ditopic Receptors....Pages 157-169
Front Matter....Pages 171-171
The Design of Molecular Artificial Sugar Sensing Systems....Pages 173-185
PCT (Photoinduced Charge Transfer) Fluorescent Molecular Sensors for Cation Recognition....Pages 187-207
Fluorometric Detection of Anion Activity and Temperature Changes....Pages 209-227
Oxygen Diffusion in Polymer Films for Luminescence Barometry Applications....Pages 229-255
Dual Lifetime Referencing (DLR) — a New Scheme for Converting Fluorescence Intensity into a Frequency-Domain or Time-Domain Information....Pages 257-274
Front Matter....Pages 275-275
Two-Photon Fluorescence Fluctuation Spectroscopy....Pages 277-296
Fluorescence Lifetime Imaging Microscopy of Signal Transduction Protein Reactions in Cells....Pages 297-302
New Techniques for DNA Sequencing Based on Diode Laser Excitation and Time-Resolved Fluorescence Detection....Pages 303-329
The Integration of Single Molecule Detection Technologies into Miniaturized Drug Screening: Current Status and Future Perspectives....Pages 331-379
Picosecond Fluorescence Lifetime Imaging Spectroscopy as a New Tool for 3D Structure Determination of Macromolecules in Living Cells....Pages 381-410
Front Matter....Pages 411-411
About the Prediction of Tryptophan Fluorescence Lifetimes and the Analysis of Fluorescence Changes in Multi-Tryptophan Proteins....Pages 413-423
Application of Time-Resolved Fluorescence Spectroscopy to Studies of DNA-Protein Interactions and RNA Folding....Pages 425-438
Front Matter....Pages 411-411
Rare Earth Cryptates and TRACE Technology as Tools for Probing Molecular Interactions in Biology....Pages 439-455
Tracking Molecular Dynamics of Flavoproteins with Time-Resolved Fluorescence Spectroscopy....Pages 457-485
Back Matter....Pages 487-490
