This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.
Author(s): Sissel Beate Rønning
Series: Methods in Molecular Biology
Publisher: Humana Press
Year: 2018
Language: English
Pages: 353
Front Matter ....Pages i-xii
Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS) (Anna Pakula, Janelle M. Spinazzola, Emanuela Gussoni)....Pages 1-15
Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells (Nataša Nikolić, Vigdis Aas)....Pages 17-24
Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD (Jingjuan Chen, Chao Wang, Shihuan Kuang)....Pages 25-41
Chromatin Immunoprecipitation in Skeletal Myoblasts (Vinay Kumar Rao, Shilpa Rani Shankar, Reshma Taneja)....Pages 43-54
Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor (Daniel C. Turner, Andreas M. Kasper, Robert A. Seaborne, Alexander D. Brown, Graeme L. Close, Mark Murphy et al.)....Pages 55-79
Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation (Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya)....Pages 81-93
Lentivirus-Mediated RNAi in Skeletal Myogenesis (Dongwook Kim, Adriana Reyes-Ordoñez, Jie Chen)....Pages 95-110
Adipogenesis from Bovine Precursors (Frea Mehta, Ruud Theunissen, Mark J. Post)....Pages 111-125
Transcriptomic Profiling During Myogenesis (Alicja Majewska, Tomasz Domoradzki, Katarzyna Grzelkowska-Kowalczyk)....Pages 127-168
Coculture Method to Obtain Endothelial Networks Within Human Tissue-Engineered Skeletal Muscle (Dacha Gholobova, Melanie Gerard, Lisanne Terrie, Linda Desender, Janet Shansky, Herman Vandenburgh et al.)....Pages 169-183
Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System (Danielle E. Dye, Beverley F. Kinnear, Vishal Chaturvedi, Deirdre R. Coombe)....Pages 185-212
LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue (Marta Moreno-Torres, Jesper F. Havelund, Nils J. Faergeman)....Pages 213-228
A siRNA Mediated Screen During C2C12 Myogenesis (Alexis Parenté, Luce Pèrié, Laetitia Magnol, Khaled Bouhouche, Véronique Blanquet)....Pages 229-243
Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury (Dhanushika Ratnayake, Peter D. Currie)....Pages 245-254
Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis (Eva Veiseth-Kent, Vibeke Høst, Mona E. Pedersen)....Pages 255-266
Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis (Anton L. Bryantsev, Lizzet Castillo, Sandy T. Oas, Maria B. Chechenova, Tracy E. Dohn, TyAnna L. Lovato)....Pages 267-281
Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers (Mark W. Pataky, Edward B. Arias, Gregory D. Cartee)....Pages 283-300
Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis (Fiona K. Jones, Gemma E. Hardman, Samantha Ferries, Claire E. Eyers, Addolorata Pisconti)....Pages 301-317
Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies (Tone-Kari K. Oestbye, Elisabeth Ytteborg)....Pages 319-330
RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis (Aynur Kaya-Çopur, Frank Schnorrer)....Pages 331-348
Back Matter ....Pages 349-351
