In this monograph, the importance of fluctuations for biological reactions is discussed from various points of view. Understanding the biological reactions at the molecular level is one of the major targets in many scientific fields, including not only basic biology but also physics, physical chemistry, and medical science. One of the key factors in the process is “fluctuation”. Thermal energy causes biological molecules to be in constant fluctuation even while they are carrying out their biological functions. How do biological systems overcome the thermal fluctuations to realize the physiologically relevant reactions? Scientists in a number of fields—physics, chemistry, pharmacology, medicine, and others—have contributed chapters that elucidate the nature of the fluctuations and the relationship between fluctuations and biological functions. The fluctuations discussed in this volume are detected by the transient grating method, nuclear magnetic resonance, X-ray diffraction and scattering, and computer simulation, among other methods. The book presents various results of the studies of fluctuations in biological processes that were obtained with these methods by the leading scientists in their fields.
Author(s): Masahide Terazima, Mikio Kataoka, Ryuichi Ueoka, Yuko Okamoto (eds.)
Edition: 1
Publisher: Springer Japan
Year: 2016
Language: English
Pages: XII, 270
Tags: Bioorganic Chemistry; Protein-Ligand Interactions; Biophysics and Biological Physics; Physical Chemistry; Computer Appl. in Life Sciences; Molecular Medicine
Front Matter....Pages i-xii
Time-Resolved Detection of Protein Fluctuations During Reactions....Pages 1-27
Pressure Perturbation: A Prime Tool to Study Conformational Substates and Volume Fluctuations of Biomolecular Assemblies....Pages 29-64
Watching a Signaling Protein Function in Real Time via Picosecond Time-Resolved Laue Crystallography....Pages 65-85
NMR Explorations of Biomolecular Systems with Rapid Conformational Exchanges....Pages 87-103
Site-Specific Incorporation of Fluorescent Nonnatural Amino Acids into Proteins and Its Application to Fluorescence Analysis of Proteins....Pages 105-123
Unfoldomes and Unfoldomics: Introducing Intrinsically Disordered Proteins....Pages 125-150
Structure, Dynamics, and Function of Staphylococcal Nuclease....Pages 151-161
Theory of Molecular Recognition and Structural Fluctuation of Biomolecules....Pages 163-181
Structural Fluctuations of Proteins in Folding and Ligand Docking Studied by Replica-Exchange Simulations....Pages 183-204
Spatiotemporal Fluctuations of Protein Folding in Living Cells....Pages 205-219
Membrane-Targeted Nanotherapy with Hybrid Liposomes for Cancer Cells Leading to Apoptosis....Pages 221-244
Antitumor Complexes Formed by Oleic Acid and Molten Globule Intermediates of Proteins....Pages 245-270
