Molecular techniques are proving invaluable in determining the phylogenetic status of potentially endangered species, for investigating mechanisms of speciation, and for measuring the genetic structure of populations. It is increasingly important for ecologists and evolutionary and conservation biologists to understand and use such molecular techniques, but most workers in these areas have not been trained in molecular biology. This book lays out the principles and basic techniques for the molecular tools appropriate for addressing issues in conservation, and it presents case studies showing how these tools have been used successfully in conservation biology. Examples include the genetic analysis of population structure, various uses of DNA in conservation genetics, and estimation of migration parameters from genetic data. Wildlife managers, as well as researchers in these areas, will find this a valuable book.
Author(s): Thomas B. Smith, Robert K. Wayne
Publisher: Oxford University Press, USA
Year: 1996
Language: English
Commentary: 8190
Pages: 498
Contents......Page 8
1. An Overview of the Issues......Page 18
I: APPROACHES......Page 38
2. Nuclear Genetic Analysis of Population Structure and Genetic Variation Using Intron Primers......Page 40
3. Application of Anonymous Nuclear Loci to Conservation Biology......Page 53
4. The Use of Random Amplified Polymorphic DNA (RAPD) in Conservation Genetics......Page 69
5. The Utility of Paternally Inherited Nuclear Genes in Conservation Genetics......Page 89
6. Applications of Allozyme Electrophoresis in Conservation Biology......Page 102
7. Regional Approaches to Conservation Biology: RFLPs, DNA Sequence, and Caribbean Birds......Page 119
8. The Use of Mitochondrial DNA Control Region Sequencing in Conservation Genetics......Page 140
9. Chloroplast DNA Sequencing to Resolve Plant Phylogenies Between Closely Related Taxa......Page 158
10. Reconstructing Population History Using PCR-Restriction Site Data......Page 169
11. The Use of PCR-Based Single-Stranded Conformation Polymorphism Analysis (PCR-SSCP) in Conservation Genetics......Page 182
12. Application of Chloroplast DNA Restriction Site Studies for Conservation Genetics......Page 198
13. A PCR Approach to Detection of Malaria in Hawaiian Birds......Page 217
14. Polymorphism of Genes in the Major Histocompatibility Complex (MHO): Implications for Conservation Genetics of Vertebrates......Page 229
15. DNA Multilocus Fingerprinting Using Simple Repeat Motif Oligonucleotides......Page 253
16. Minisatellite Analysis in Conservation Genetics......Page 266
17. Microsatellites and Their Application to Conservation Genetics......Page 293
18. Noninvasive Genotyping for Vertebrate Conservation......Page 313
19. Future Applications of PCR to Conservation Biology......Page 329
II: ANALYSIS......Page 342
20. Estimation of Effective Population Size and Migration Parameters from Genetic Data......Page 344
21. Simulation Models of Bottleneck Events in Natural Populations......Page 362
22. Assessing Relatedness and Evolutionary Divergence: Why the Genetic Evidence Alone Is Insufficient......Page 380
III: CASE STUDIES......Page 396
23. Molecular Genetics and the Conservation of Salmonid Biodiversity: Oncorhynchus at the Edge of Their Range......Page 398
24. Population Genetics of Kenyan Impalas—Consequences for Conservation......Page 414
25. Paternity Studies in Animal Populations......Page 428
26. Genetic Structure of Natural Taxus Populations in Western North America......Page 439
27. Applications of Genetics to the Conservation and Management of Australian Fauna: Four Case Studies from Queensland......Page 457
IV: PERSPECTIVE......Page 472
28. Conservation Genetics and Molecular Techniques: A Perspective......Page 474
C......Page 493
I......Page 494
M......Page 495
P......Page 496
U......Page 497
Z......Page 498
