Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations.
Author(s): Jacqueline Boultwood, Carrie Fidler
Series: Methods in Molecular Medicine
Edition: 1st
Publisher: Humana Press
Year: 2001
Language: English
Pages: 302
Preface......Page 6
Contents......Page 8
Molecular Analysis of Cancer: An Overview......Page 13
Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization......Page 18
Spectral Karyotyping in Cancer Cytogenetics......Page 39
Comparative Genomic Hybridization Analysis......Page 55
Detection of Chromosomal Deletions by Microsatellite Analysis......Page 68
Detection and Quantification of Leukemia-Specific Rearrangements......Page 75
Detection of t(2;5)(p23;q35) Translocation by Long-Range PCR of Genomic DNA......Page 105
Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer......Page 114
Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism......Page 122
Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis......Page 132
Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay......Page 147
Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay......Page 162
Mutation Analysis of Cancer Using Automated Sequencing......Page 176
Detection of Differentially Expressed Genes in Cancer Using Differential Display......Page 183
Genomewide Gene Expression Analysis Using cDNA Microarrays......Page 198
Gene Expression Profiling in Cancer Using cDNA Microarrays......Page 208
Wilms Tumor Gene WT1 as a Tumor Marker for Leukemic Blast Cells and Its Role in Leukemogenesis......Page 226
Detection of Aberrant Methylation of the p15INK4B Gene Promoter......Page 240
Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon......Page 251
Telomere Length Changes in Human Cancer......Page 271
Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders......Page 279
Index......Page 300
