This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.
Author(s): Liza A. Pon and Eric A. Schon (Eds.)
Series: Methods in Cell Biology 80
Edition: 2
Publisher: Academic Press
Year: 2007
Language: English
Commentary: +OCR
Pages: 1-920
Content:
Isolation and Subfractionation of Mitochondria from Animal Cells and Tissue Culture Lines Review Article
Pages 3-44
Francesco Pallotti, Giorgio Lenaz
Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae Review Article
Pages 45-64
Istvan R. Boldogh, Liza A. Pon
Isolation and Subfractionation of Mitochondria from Plants Review Article
Pages 65-90
A.H. Millar, A. Liddell, C.J. Leaver
Biochemical Assays of Respiratory Chain Complex Activity Review Article
Pages 93-119
Denise M. Kirby, David R. Thorburn, Douglass M. Turnbull, Robert W. Taylor
Polarographic Assays of Respiratory Chain Complex Activity* Review Article
Pages 121-133
Gaetano Villani, Giuseppe Attardi
Optical Imaging Techniques (Histochemical, Immunohistochemical, and In Situ Hybridization Staining Methods) to Visualize Mitochondria Review Article
Pages 135-154
Kurenai Tanji, Eduardo Bonilla
Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues Review Article
Pages 155-171
Cristofol Vives‐Bauza, Lichuan Yang, Giovanni Manfredi
Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures Review Article
Pages 173-178
Nevi Mackay, Brian H. Robinson
Assays of Fatty Acid β‐Oxidation Activity Review Article
Pages 179-197
Michael J. Bennett
Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc Review Article
Pages 199-222
Ann Saada Reisch, Orly Elpeleg
Assays of Cardiolipin Levels Review Article
Pages 223-240
Michael Schlame
Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems Review Article
Pages 241-260
Marco Colombini
Methods for Studying Iron Metabolism in Yeast Mitochondria Review Article
Pages 261-280
Sabine Molik, Roland Lill, Ulrich Mühlenhoff
Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores Review Article
Pages 283-295
John J. Lemasters, Venkat K. Ramshesh
Biosensors for the Detection of Calcium and pH Review Article
Pages 297-325
Paolo Pinton, Alessandro Rimessi, Anna Romagnoli, Andrea Prandini, Rosario Rizzuto
Measurement of Membrane Permeability and the Permeability Transition of Mitochondria Review Article
Pages 327-340
Naoufal Zamzami, Carine Maisse, Didier Métivier, Guido Kroemer
Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes Review Article
Pages 341-352
Christopher J. Bell, Giovanni Manfredi, Elinor J. Griffiths, Guy A. Rutter
Measurement of Reactive Oxygen Species in Cells and Mitochondria Review Article
Pages 355-377
J.S. Armstrong, M. Whiteman
Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase Review Article
Pages 379-393
Cristofol Vives‐Bauza, Anatoly Starkov, Elena Garcia‐Arumi
Methods for Measuring the Regulation of Respiration by Nitric Oxide Review Article
Pages 395-416
Sruti Shiva, Paul S. Brookes, Victor M. Darley‐Usmar
Methods for Determining the Modification of Protein Thiols by Reactive Lipids Review Article
Pages 417-434
JooYeun Oh, Michelle S. Johnson, Aimee Landar
Detection of Mutations in mtDNA Review Article
Pages 437-463
Ali Naini, Sara Shanske
Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans Review Article
Pages 465-479
Gerald S. Shadel, Bonnie L. Seidel‐Rogol
Microdissection and Analytical PCR for the Investigation of mtDNA Lesions Review Article
Pages 481-501
Sion L. Williams, Carlos T. Moraes
Transmitochondrial Technology in Animal Cells Review Article
Pages 503-524
Sandra R. Bacman, Carlos T. Moraes
Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii Mitochondria Review Article
Pages 525-548
Nathalie Bonnefoy, Claire Remacle, Thomas D. Fox
Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases Review Article
Pages 549-569
Carl A. Pinkert, Ian A. Trounce
In Vivo and In Organello Analyses of Mitochondrial Translation Review Article
Pages 571-588
P. Fernández‐Silva, R. Acín‐Pérez, E. Fernández‐Vizarra, A. Pérez‐Martos, J.A. Enriquez
Visualization of Mitochondria in Budding Yeast Review Article
Pages 591-626
Theresa C. Swayne, Anna C. Gay, Liza A. Pon
Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells Review Article
Pages 627-682
Kurt J. De Vos, Michael P. Sheetz
Cell‐Free Assays for Mitochondria–Cytoskeleton Interactions Review Article
Pages 683-706
Istvan R. Boldogh, Liza A. Pon, Michael P. Sheetz, Kurt J. De Vos
In Vitro Assays for Mitochondrial Fusion and Division Review Article
Pages 707-720
Elena Ingerman, Shelly Meeusen, Rachel DeVay, Jodi Nunnari
Electrophoretic Methods to Isolate Protein Complexes from Mitochondria Review Article
Pages 723-741
Ilka Wittig, Hermann Schägger
Analysis of Protein–Protein Interactions in Mitochondria Review Article
Pages 743-759
Johannes M. Herrmann, Benedikt Westermann
Analysis and Prediction of Mitochondrial Targeting Signals Review Article
Pages 761-781
Shukry J. Habib, Walter Neupert, Doron Rapaport
Import of Proteins into Mitochondria Review Article
Pages 783-806
Diana Stojanovski, Nikolaus Pfanner, Nils Wiedemann
Series Editors
Page II
Contents
Pages V-XV
Contributors to Volume 80
Pages XVII-XXI
Preface
Pages XXIII-XXIV
Liza A. Pon, Eric A. Schon
Appendix 1. Basic Properties of Mitochondria Review Article
Pages 809-812
Luis J. García‐Rodríguez
Appendix 2. Direct and Indirect Inhibitors of Mitochondrial ATP Synthesis Review Article
Pages 813-826
Nanette Orme‐Johnson
Appendix 3. Linearized Maps of Circular Mitochondrial Genomes from Representative Organisms Review Article
Pages 827-829
Eric A. Schon
Appendix 4. Mitochondrial Genetic Codes in Various Organisms Review Article
Pages 831-833
Compiled by Eric A. Schon
Appendix 5. Gene Products Present in Mitochondria of Yeast and Animal Cells Review Article
Pages 835-876
Eric A. Schon
Appendix 6. Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions Review Article
Pages 877-887
Eric A. Schon
Index
Pages 889-911
Volumes in Series
Pages 913-920
