Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Author(s): Lyne Jossé, Lin Zhang, C Mark Smales (auth.), Niall Barron (eds.)
Edition: 1
Publisher: Springer Netherlands
Year: 2012
Language: English
Pages: 126
Tags: Pharmaceutical Sciences/Technology; Genetic Engineering; Animal Genetics and Genomics
Front Matter....Pages 1-1
A General Introduction to MicroRNAs, Their Investigation and Exploitation in CHO Cell Lines....Pages 1-13
Biogenesis of Mammalian miRNA....Pages 15-27
Target Prediction Algorithms and Bioinformatics Resources for miRNA Studies....Pages 29-48
The CHO miRNA Transcriptome....Pages 49-64
MicroRNAs as Engineering Targets: Pathway Manipulation to Impact Bioprocess Phenotypes....Pages 65-85
MicroRNAs for Enhancement of CHO Cell Proliferation and Stability: Insights from Neuroblastoma Studies....Pages 87-97
MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production....Pages 99-111
Manipulating Levels of Specific MicroRNAs in Mammalian Cells....Pages 113-121
Back Matter....Pages 119-119
