MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.
Author(s): Shao-Yao Ying
Series: Methods in Molecular Biology
Edition: 1
Publisher: Humana Press
Year: 2006
Language: English
Pages: 381
頁面擷取自 342-MicroRNA Protocols.pdf......Page 1
342-MicroRNA Protocols.pdf......Page 2
MicroRNA Protocols......Page 4
Preface......Page 6
Contents......Page 10
Contributors......Page 12
1. The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions......Page 16
2. Structure Analysis of MicroRNA Precursors......Page 34
3. MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex......Page 48
4. Recognition and Cleavage of Primary MicroRNA Transcripts......Page 64
5. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery......Page 72
6. MicroRNAs and Messenger RNA Turnover......Page 88
7. Prediction of MicroRNA Targets......Page 102
8. Prediction of Human MicroRNA Targets......Page 116
9. Complications in Mammalian MicroRNA Target Prediction......Page 130
10. miRBase:The MicroRNA Sequence Database......Page 144
11. Methodologies for High-Throughput Expression Profiling of MicroRNAs......Page 154
12. In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development......Page 174
13. Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA......Page 196
14. Cloning MicroRNAs From Mammalian Tissues......Page 204
15. Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation......Page 224
16. Identifying MicroRNA Regulators of Cell Death in Drosophila......Page 244
17. MicroRNAs in Human Immunodeficiency Virus-1 Infection......Page 256
18. Cloning and Detection of HIV-1-Encoded MicroRNA......Page 270
19. Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein......Page 282
20. In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates......Page 292
21. Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector......Page 302
22. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs......Page 310
23. Isolation and Identification of Gene-Specific MicroRNAs......Page 328
24. Transgene-Like Animal Models Using Intronic MicroRNAs......Page 336
25. Evolution of MicroRNAs......Page 350
26. Perspectives......Page 366
Index......Page 374
