This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.
Author(s): Debashis Dutta
Series: Methods in Molecular Biology
Publisher: Humana Press
Year: 2019
Language: English
Pages: 259
Front Matter ....Pages i-x
Fabrication of Glass Microfluidic Devices (Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, Shu Jia)....Pages 1-12
Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices (Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, Sk Rameez Iqbal)....Pages 13-54
Sample Injection Techniques (James M. Karlinsey)....Pages 55-64
Sample Preconcentration Protocols in Microfluidic Electrophoresis (Fumihiko Kitagawa, Koji Otsuka)....Pages 65-78
Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection (Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, José Alberto Fracassi da Silva)....Pages 79-85
Micellar Electrokinetic Chromatography (Braden C. Giordano, Ronald Siefert, Greg E. Collins)....Pages 87-97
Microchip Isotachophoresis: Analysis of Pharmaceuticals (Marián Masár, Jasna Hradski)....Pages 99-111
Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination (Stefan Nagl)....Pages 113-124
Nanochannel Gradient Separations (Michael A. Startsev, David W. Inglis)....Pages 125-132
Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications (Cyro L. S. Chagas, Thiago M. G. Cardoso, Wendell K. T. Coltro)....Pages 133-142
Band Broadening Theories in Capillary Electrophoresis (Sandip Ghosal)....Pages 143-166
Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation (Debashis Dutta)....Pages 167-195
Microchip Electrophoresis Tools for the Analysis of Small Molecules (Federico J. V. Gomez, María Fernanda Silva)....Pages 197-206
Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS)—Swab Sample-In to DNA Profile-Out (Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, Frederic Zenhausern)....Pages 207-224
Achieving Stable Electrospray Ionization Mass Spectrometry Detection from Microfluidic Chips (Iulia M. Lazar)....Pages 225-237
Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow (Ling Xia, Debashis Dutta)....Pages 239-249
Back Matter ....Pages 251-255
