Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages. * * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.
Author(s): Kenneth W. Adolph (Eds.)
Series: Methods in Molecular Genetics 6
Edition: 1
Publisher: Elsevier, Academic Press
Year: 1995
Language: English
Pages: 3-487
Content:
Contributors to Volume 6
Pages ix-xiv
Preface
Page xv
Kenneth W. Adolph
Methods in molecular genetics
Page xvii
[1] Forward and reverse genetics in the study of the obligate, intracellular parasite Toxoplasma gondii Original Research Article
Pages 3-29
John C. Boothroyd, Michael Black, Kami Kim, Elmer R. Pfefferkorn, Frank Seeber, David Sibley, Dominique Soldati
[2] Techniques associated with protozoan parasite genome analysis Original Research Article
Pages 30-47
James W. Ajioka, Sara E. Melville, Richard M.R. Coulson, Jose R. Espinoza, Shahid M. Khan, Kiew-Lian Wan
[3] Aspergillus nidulans as a model system for cell and molecular biology studies Original Research Article
Pages 48-65
Gustavo H. Golduran, N. Ronald Morris
[4] Special molecular techniques for study of filamentous fungi Original Research Article
Pages 66-78
George A. Marzluf
[5] In Vitro fragmentation of yeast chromosomes and yeast artificial chromosomes at artificially inserted sites and applications to genome mapping Original Research Article
Pages 81-107
Hervé Tettelin, Agnès Thierry, Cécile Fairhead, Arnaud Perrin, Bernard Dujon
[6] Basic analysis of transcription factor binding to nucleosomes Original Research Article
Pages 108-128
Jacques Côté, Rhea T. Utley, Jerry L. Workman
[7] Experimental analysis of transcription factor-nucleosome interactions Original Research Article
Pages 129-152
Michelle Vettese-Dadey, Christopher C. Adams, Jacques Côté, Phillip Walter, Jerry L. Workman
[8] In Vivo Analysis of nucleosome structure and transcription factor binding in Saccharomyces cerevisiae Original Research Article
Pages 153-167
John Svaren, Ulrike Venter, Wolfram Hörz
[9] Mapping of yeast nucleosomes in Vivo Original Research Article
Pages 168-185
Memmo Buttinelli, Giorgio Camilloni, Giovanna Costanzo, Rodolfo Negri, Patrizia Venditti, Sabrina Venditti, Ernesto Di Mauro
[10] Novel assays for ligation and cleavage activities of site-specific recombinases Original Research Article
Pages 186-203
Paul D. Sadowski, Guohua Pan, Roland Brousseau
[11] Physical monitoring of mitotic and meiotic recombination in Saccharomyces cerevisiae Original Research Article
Pages 204-226
James E. Haber, Neal Sugawara
[12] Genetic dissection of complex heteromultimeric enzymes: The case of yeast DNA-dependent RNA polymerases Original Research Article
Pages 227-246
Pierre Thuriaux, Michel Werner, Sophie Stettler, Dominique Lalo
[13] Pheromone procedures in fission yeast Original Research Article
Pages 247-263
John Davey, Richard Egel, Olaf Nielsen
[14] Use of DNA Methylation Deficient Strains in Molecular Genetics Original Research Article
Pages 267-279
Lene Juel Rasmussen, M.G. Marinus
[15] Identifying ends of infrequent RNA molecules in bacteria Original Research Article
Pages 280-300
Vincent J. Cannistraro, David Kennell
[16] Identification of rcs Genes in Escherichia coli O9:K30:H12 and Involvement in Regulation of Expression of Group IA K30 Capsular Polysaccharide Original Research Article
Pages 301-322
Chris Whitfield, Wendy J. Keenleyside, P. Ronald MacLachlan, Padman Jayaratne, Anthony J. Clarke
[17] Genetic mapping in Bacillus subtilis Original Research Article
Pages 323-338
Simon M. Cutting, Vasco Azevedo
[18] Molecular and Biochemical Methods for Studying Chemotaxis in Bacillus subtilis Original Research Article
Pages 339-366
Mia Mae L. Rosario, George W. Ordal
[19] Cloning of Thermomonospora fusca Cellulase Genes in Escherichia coli and Streptomyces lividans Original Research Article
Pages 367-374
David B. Wilson
[20] Computational Analyses Aiding Identification and Characterization of Proteins, Genes, and Operons Original Research Article
Pages 375-386
Milton H. Saier Jr., Jonathan Reizer
[21] Techniques to isolate specialized templates for in Vitro transcription assays Original Research Article
Pages 389-399
Susan Garges, Hyon E. Choy, Sangryeol Ryu
[22] Protein-protein interactions of DNA-binding proteins: Studies on replication initiator protein, RepA, of plasmid P1 Original Research Article
Pages 400-420
Gauranga Mukhopadhyay, Justin A. Dibbens, Dhruba K. Chattoraj
[23] Transcriptional regulators: Protein-DNA complexes and regulatory mechanisms Original Research Article
Pages 421-438
Fernando Rojo, Margarita Salas
[24] Use of coliphage λ and other bacteriophages for molecular genetic analysis of Erwinia and related gram-negative bacteria Original Research Article
Pages 439-454
Vincent Mulholland, George P.C. Salmond
[25] Isolation, purification, and function of assembly intermediates and subviral particles of bacteriophages PRD1 and σ6 Original Research Article
Pages 455-474
Dennis H. Bamford, Päivi M. Ojala, Mikko Frilander, Laura Walin, Jaana K.H. Bamford
Index
Pages 475-487
