Microbial Gene Techniques

Content:
Contributors to Volume 6
Pages ix-xiv

Preface
Page xv
Kenneth W. Adolph

Methods in molecular genetics
Page xvii

[1] Forward and reverse genetics in the study of the obligate, intracellular parasite Toxoplasma gondii Original Research Article
Pages 3-29
John C. Boothroyd, Michael Black, Kami Kim, Elmer R. Pfefferkorn, Frank Seeber, David Sibley, Dominique Soldati

[2] Techniques associated with protozoan parasite genome analysis Original Research Article
Pages 30-47
James W. Ajioka, Sara E. Melville, Richard M.R. Coulson, Jose R. Espinoza, Shahid M. Khan, Kiew-Lian Wan

[3] Aspergillus nidulans as a model system for cell and molecular biology studies Original Research Article
Pages 48-65
Gustavo H. Golduran, N. Ronald Morris

[4] Special molecular techniques for study of filamentous fungi Original Research Article
Pages 66-78
George A. Marzluf

[5] In Vitro fragmentation of yeast chromosomes and yeast artificial chromosomes at artificially inserted sites and applications to genome mapping Original Research Article
Pages 81-107
Hervé Tettelin, Agnès Thierry, Cécile Fairhead, Arnaud Perrin, Bernard Dujon

[6] Basic analysis of transcription factor binding to nucleosomes Original Research Article
Pages 108-128
Jacques Côté, Rhea T. Utley, Jerry L. Workman

[7] Experimental analysis of transcription factor-nucleosome interactions Original Research Article
Pages 129-152
Michelle Vettese-Dadey, Christopher C. Adams, Jacques Côté, Phillip Walter, Jerry L. Workman

[8] In Vivo Analysis of nucleosome structure and transcription factor binding in Saccharomyces cerevisiae Original Research Article
Pages 153-167
John Svaren, Ulrike Venter, Wolfram Hörz

[9] Mapping of yeast nucleosomes in Vivo Original Research Article
Pages 168-185
Memmo Buttinelli, Giorgio Camilloni, Giovanna Costanzo, Rodolfo Negri, Patrizia Venditti, Sabrina Venditti, Ernesto Di Mauro

[10] Novel assays for ligation and cleavage activities of site-specific recombinases Original Research Article
Pages 186-203
Paul D. Sadowski, Guohua Pan, Roland Brousseau

[11] Physical monitoring of mitotic and meiotic recombination in Saccharomyces cerevisiae Original Research Article
Pages 204-226
James E. Haber, Neal Sugawara

[12] Genetic dissection of complex heteromultimeric enzymes: The case of yeast DNA-dependent RNA polymerases Original Research Article
Pages 227-246
Pierre Thuriaux, Michel Werner, Sophie Stettler, Dominique Lalo

[13] Pheromone procedures in fission yeast Original Research Article
Pages 247-263
John Davey, Richard Egel, Olaf Nielsen

[14] Use of DNA Methylation Deficient Strains in Molecular Genetics Original Research Article
Pages 267-279
Lene Juel Rasmussen, M.G. Marinus

[15] Identifying ends of infrequent RNA molecules in bacteria Original Research Article
Pages 280-300
Vincent J. Cannistraro, David Kennell

[16] Identification of rcs Genes in Escherichia coli O9:K30:H12 and Involvement in Regulation of Expression of Group IA K30 Capsular Polysaccharide Original Research Article
Pages 301-322
Chris Whitfield, Wendy J. Keenleyside, P. Ronald MacLachlan, Padman Jayaratne, Anthony J. Clarke

[17] Genetic mapping in Bacillus subtilis Original Research Article
Pages 323-338
Simon M. Cutting, Vasco Azevedo

[18] Molecular and Biochemical Methods for Studying Chemotaxis in Bacillus subtilis Original Research Article
Pages 339-366
Mia Mae L. Rosario, George W. Ordal

[19] Cloning of Thermomonospora fusca Cellulase Genes in Escherichia coli and Streptomyces lividans Original Research Article
Pages 367-374
David B. Wilson

[20] Computational Analyses Aiding Identification and Characterization of Proteins, Genes, and Operons Original Research Article
Pages 375-386
Milton H. Saier Jr., Jonathan Reizer

[21] Techniques to isolate specialized templates for in Vitro transcription assays Original Research Article
Pages 389-399
Susan Garges, Hyon E. Choy, Sangryeol Ryu

[22] Protein-protein interactions of DNA-binding proteins: Studies on replication initiator protein, RepA, of plasmid P1 Original Research Article
Pages 400-420
Gauranga Mukhopadhyay, Justin A. Dibbens, Dhruba K. Chattoraj

[23] Transcriptional regulators: Protein-DNA complexes and regulatory mechanisms Original Research Article
Pages 421-438
Fernando Rojo, Margarita Salas

[24] Use of coliphage λ and other bacteriophages for molecular genetic analysis of Erwinia and related gram-negative bacteria Original Research Article
Pages 439-454
Vincent Mulholland, George P.C. Salmond

[25] Isolation, purification, and function of assembly intermediates and subviral particles of bacteriophages PRD1 and σ6 Original Research Article
Pages 455-474
Dennis H. Bamford, Päivi M. Ojala, Mikko Frilander, Laura Walin, Jaana K.H. Bamford

Index
Pages 475-487