Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
Author(s): Stephen M. King and Gregory J. Pazour (Eds.)
Series: Methods in Cell Biology 91
Edition: 1
Publisher: Academic Press
Year: 2009
Language: English
Pages: 1-332
Tags: Биологические дисциплины;Цитология;
Content:
Series Editors
Page ii
Volume Editors
Page iii
Copyright
Page iv
Contributors
Pages ix-x
Preface
Pages xi-xii
Chapter 1 - Cryo-Electron Microscope Tomography to Study Axonemal Organization
Pages 1-39
Daniela Nicastro
Chapter 2 - Electron Microscopic Imaging and Analysis of Isolated Dynein Particles
Pages 41-61
Anthony J. Roberts, Stan A. Burgess
Chapter 3 - Immunogold Labeling of Flagellar Components In Situ
Pages 63-80
Stefan Geimer
Chapter 4 - Scanning Electron Microscopy to Examine Cells and Organs
Pages 81-87
Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, Gregory J. Pazour
Chapter 5 - X-ray Fiber Diffraction Studies on Flagellar Axonemes
Pages 89-109
Kazuhiro Oiwa, Shinji Kamimura, Hiroyuki Iwamoto
Chapter 6 - Markers for Neuronal Cilia
Pages 111-121
Jacqueline S. Domire, Kirk Mykytyn
Chapter 7 - Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells
Pages 123-133
Heymut Omran, Niki T. Loges
Chapter 8 - Immunoprecipitation to Examine Protein Complexes
Pages 135-142
Gregory J. Pazour
Chapter 9 - Tandem Affinity Purification of Ciliopathy-Associated Protein Complexes
Pages 143-160
Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner, Marius Ueffing, Ronald Roepman
Chapter 10 - Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products
Pages 161-171
Miho Sakato
Chapter 11 - Analysis of the Ciliary/Flagellar Beating of Chlamydomonas
Pages 173-239
Kenneth W. Foster
Chapter 12 - Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii
Pages 241-253
Ritsu Kamiya
Chapter 13 - High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain
Pages 255-264
Karl-Ferdinand Lechtreck, Michael J. Sanderson, George B. Witman
Chapter 14 - Observation of Nodal Cilia Movement and Measurement of Nodal Flow
Pages 265-285
Yasushi Okada, Nobutaka Hirokawa
Chapter 15 - Modification of Mouse Nodal Flow by Applying Artificial Flow
Pages 287-297
Shigenori Nonaka
Chapter 16 - Measuring Cilium-Induced Ca2+ Increases in Cultured Renal Epithelia
Pages 299-313
Helle A. Praetorius
Index
Pages 315-323
Volume in Series
Pages 325-332
Color Plates
Pages C1-C16
