Membrane protein - Expression, purification and characterization

Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli......Page 2
Dihydroorotate Dehydrogenase of Escherichia coli......Page 10
A General Approach for Heterologous Membrane Protein Expression in Escherichia coli......Page 21
Expression of Membrane-Bound Iron–Sulfur Proteins......Page 35
Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris......Page 47
Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells......Page 63
Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells......Page 72
Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution......Page 87
Solubilization of Chemokine Receptors from Cell Membranes......Page 101
A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid......Page 109
Purification of Omp50, a Minor Porin of Campylobacter jejuni......Page 127
Purification of Porins from Mycobacterium smegmatis......Page 136
Isolation of the Melanocortin 5 Receptor......Page 149
Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography......Page 161
Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells......Page 174
Purification of Pancreastatin Receptor from Rat Liver Membranes......Page 187
Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes......Page 196
Isolation of Lipid Raft-Associated Proteolipids......Page 224
Purification of Membrane-Bound Catechol-O-Methyltransferase......Page 233
Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane......Page 242
Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase......Page 260
Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes......Page 274
Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I......Page 284
Crystallization in Lipidic Cubic Phases......Page 306
Optical Biosensor Assay Using Retroviral Receptor Pseudotypes......Page 319