Membrane Hydration: The Role of Water in the Structure and Function of Biological Membranes

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This book is about the importance of water in determining the structure, stability and responsive behavior of biological membranes. Water confers to lipid membranes unique features in terms of surface and mechanical properties. The analysis of the hydration forces, plasticiser effects, controlled hydration, formation of microdomains of confined water suggests that water is an active constituent in a water-lipid system.

The chapters describe water organization at the lipid membrane–water interphase, the water penetration, the long range water structure in the presence of lipid membranes by means of X-ray and neutron scattering, general polarization, fluorescent probes, ATR-FTIR and near infrared spectroscopies, piezo electric methods, computer simulation and surface thermodynamics.

Permeation, percolation, osmotic stress, polarization, protrusion, sorption, hydrophobicity, density fluctuations are treated in detail in self-assembled bilayers. Studies in lipid monolayers show the correlation of surface pressure with water activity and its role in peptide and enzyme interactions. The book concludes with a discussion on anhydrobiosis and the effect of water replacement in microdomains and its consequence for cell function.

New definitions of lipid/water interphases consider water not only as a structural-making solvent but as a mediator in signalling metabolic activity, modulating protein insertion and enzymatic activity, triggering oscillatory reactions and functioning of membrane bound receptors. Since these effects occur at the molecular level, membrane hydration appears fundamental to understand the behavior of nano systems and confined environments mimicking biological systems.

These insights in structural, thermodynamical and mechanical water properties give a base for new paradigms in membrane structure and function for those interested in biophysics, physical chemistry, biology, bio and nano medicine, biochemistry,

biotechnology and nano sciences searching for biotechnological inputs in human health, food industry, plant growing and energy conversion.

Author(s): E. Anibal Disalvo (eds.)
Series: Subcellular Biochemistry 71
Edition: 1
Publisher: Springer International Publishing
Year: 2015

Language: English
Pages: XVIII, 284
Tags: Membrane Biology; Biomedicine general; Physical Chemistry

Front Matter....Pages i-xviii
Membrane Hydration: A Hint to a New Model for Biomembranes....Pages 1-16
Use of X-Ray and Neutron Scattering Methods with Volume Measurements to Determine Lipid Bilayer Structure and Number of Water Molecules/Lipid....Pages 17-43
Water and Lipid Bilayers....Pages 45-67
Hydration Forces Between Lipid Bilayers: A Theoretical Overview and a Look on Methods Exploring Dehydration....Pages 69-104
Monitoring Membrane Hydration with 2-(Dimethylamino)-6-Acylnaphtalenes Fluorescent Probes....Pages 105-125
Long-Range Lipid-Water Interaction as Observed by ATR-FTIR Spectroscopy....Pages 127-159
Hydration and Nanoconfined Water: Insights from Computer Simulations....Pages 161-187
Aquaphotomics: Near Infrared Spectroscopy and Water States in Biological Systems....Pages 189-211
Hydration in Lipid Monolayers: Correlation of Water Activity and Surface Pressure....Pages 213-231
Water at Biological Phase Boundaries: Its Role in Interfacial Activation of Enzymes and Metabolic Pathways....Pages 233-261
Anhydrobiosis: An Unsolved Problem with Applications in Human Welfare....Pages 263-280
Back Matter....Pages 281-284