This manual collects in the form of laboratory protocols a series of experiments in the field of Membrane Transport and Membrane Bioenergetics. It represents the experience accumulated during four advanced courses held at the Depart ment of Biochemistry of the Swiss Federal Institute of Technology on behalf of Federation of European Biochemical Societies (FEBS) in the years 1975 through 1978. The idea of collecting the experiments into a laboratory manual developed as a response to a demand from the students who took part in the courses. Further motivation came with the fmding that, in planning the laboratory sessions, the teaching staff had no organized, modern source of information in the literature. The experiments presented cover most areas of importance in the subject mat ter. Their presentation has been continuously modified in the course of the four years during which the manual took shape, to accommodate to experience and various suggestions. In their present form, all of the experiments described have been repeatedly practiced to optimize their execution. Efforts have been made to combine in the manual classical experiments, and techniques which require relatively unsophisticated instrumentation and can therefore be carried out in most laboratories, with more modern experiments and relatively newer technol ogies. In its present form, the manual should therefore provide a usefui tool in the hands of researchers and laboratory teachers at different levels of sophisti cation and instrumentation.
Author(s): Markus Kessler, Gerhard Toggenburger (auth.), Ernesto Carafoli, Giorgio Semenza (eds.)
Edition: 1
Publisher: Springer-Verlag Berlin Heidelberg
Year: 1979
Language: English
Pages: 178
Tags: Life Sciences, general; Biomedicine general
Front Matter....Pages i-x
Nonelectrolyte Transport in Small Intestinal Membrane Vesicles. The Application of Filtration for Transport and Binding Studies....Pages 1-24
Transport of Sugars in Bacteria....Pages 25-35
Net Na + and K + Movements in Human Red Blood Cells After Cold Storage....Pages 36-40
Calcium Transport in Resealed Erythrocytes and the Use of a Calcium-Sensitive Electrode....Pages 41-50
Calcium Transport in Sarcoplasmic Reticulum Vesicles Isolated from Rabbit Skeletal Muscle....Pages 51-61
Preparation and Assay of Animal Mitochondria and Submitochondrial Vesicles....Pages 62-76
Measurement of Cytochrome Kinetics in Rat Liver Mitochondria by Stopped Flow and Dual-Wavelength Spectrophotometry....Pages 77-81
Proton Translocation Catalyzed by Mitochondrial Cytochrome Oxidase....Pages 82-91
Determination of the Membrane Potential and pH Difference Across the Inner Mitochondrial Membrane....Pages 92-97
Anion Transport in Mitochondria....Pages 98-102
Calcium Transport in Mitochondria....Pages 103-112
Redox Intermediates Between O 2 and H 2 O....Pages 113-119
Photophosphorylation with Chromatophore Membranes from Rhodospirillum Rubrum....Pages 120-143
Oxygen Evolution and Uptake as a Measure of the Light-Induced Electron Transport in Spinach Chloroplasts....Pages 144-153
The Function of the Purple Membrane in Halobacterium Halobium ....Pages 154-163
Characterization of Ionophores Using Artificial Lipid Membranes....Pages 164-170
Characterization of Neutral and Charged Ionophores Using Vesicular Artificial Lipid Membranes (Liposomes)....Pages 171-175
