This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures. Key Features* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies* Includes overviews of* Optical probes and reagents* Fabrication and use of ion-selective microelectrodes* Use of NMR spectroscopy* Ionophores
Author(s): Jacob Kraicer and S. Jeffrey Dixon (Eds.)
Series: Methods in Neurosciences 27
Edition: 1
Publisher: Elsevier, Academic Press
Year: 1995
Language: English
Pages: 1-400
Content:
Contributors to volume 27
Pages ix-xi
Preface
Pages xiii-xiv
Jacob Kraicer, S. Jeffrey Dixon
Methods in neurosciences
Pages xv-xvi
[1] Light-optical-based reagents for the measurement and manipulation of ions, metabolites, and macromolecules in living cells Original Research Article
Pages 1-16
Kenneth A. Giuliano, D. Lansing Taylor
[2] Use of ion-selective microelectrodes for recording intracellular ion levels Original Research Article
Pages 17-38
Mary E. Morris
[3] Use of NMR spectroscopy to measure intracellular ion concentrations Original Research Article
Pages 39-51
Stephen J. Karlik
[4] Use of ionophores for manipulating intracellular ion concentrations Original Research Article
Pages 52-68
G. Andrew Woolley, Douglas R. Pfeiffer, Charles M. Deber
[5] Fluorescence imaging of cytosolic calcium: An introduction to basic experimental principles Original Research Article
Pages 69-80
David A. Williams
[6] Fluorescence measurements of cytosolic calcium: Combined photometry with electrophysiology Original Research Article
Pages 81-122
W.T. Mason, J. Dempster, R. Zorec, John Hoyland, P.M. Lledo
[7] Use of chelators and photoactivatablecaged compounds to manipulate cytosolic calcium Original Research Article
Pages 123-152
Alison M. Gurney, Susan E. Bates
[8] Measurement of cytosolic calcium by 19F NMR Original Research Article
Pages 153-195
Gerry A. Smith, Herman S. Bachelard
[9] Fluorescence measurement of cytosolic pH in cultured rodent astrocytes Original Research Article
Pages 196-213
S. Jeffrey Dixon, John X. Wilson
[10] Brain cell acid-base changes measured with ion-selective microelectrodes Original Research Article
Pages 214-232
Christopher D. Lascola, Richard P. Kraig
[11] Measurements of cytosolic pH by nuclear magnetic resonance spectroscopy Original Research Article
Pages 233-251
Ognen A.C. Petroff, James W. Prichard
[12] Manipulation and regulation ofcytosolic pH Original Research Article
Pages 252-273
Mark O. Bevensee, Walter F. Boron
[13] Fluorescence measurements of cytosolicsodium concentration Original Research Article
Pages 274-288
Marli A. Robertson, J. Kevin Foskett
[14] Measurement of cytosolic sodium using ion-selective microelectrodes Original Research Article
Pages 289-303
Joachim W. Deitmer, Thomas Munsch
[15] A practical guide to the use of fluorescent indicators for the measurement of cytosolic free magnesium Original Research Article
Pages 304-318
Elizabeth Murphy, Robert E. London
[16] Measurement of cytosolic magnesium by 31P NMR spectroscopy Original Research Article
Pages 319-327
J.A. Helpern, Herbert R. Halvorson
[17] Chloride-sensitive fluorescent indicators Original Research Article
Pages 328-339
A.S. Verkman, Joachim Biwersi
[18] Measurement of cytosolic chloride activityby ion-selective microelectrodes Original Research Article
Pages 340-360
Jonathan A. Coles
[19] Use of ion-selective microelectrodes andfluorescent probes to measure cell volume Original Research Article
Pages 361-391
Francisco J. Alvarez-Leefmans, Julio Altamirano, William E. Crowe
Index
Pages 393-400
