This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics.
A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.
Author(s): Qian Wu, Lynn Sibanda, Takashi Ochi (auth.), Maria Armenia Carrondo, Paola Spadon (eds.)
Series: NATO Science for Peace and Security Series A: Chemistry and Biology
Edition: 1
Publisher: Springer Netherlands
Year: 2012
Language: English
Pages: 208
Tags: Crystallography; Protein-Ligand Interactions; Protein Structure; Simulation and Modeling; Bioinformatics
Front Matter....Pages i-xiv
Spatial and Temporal Organisation of Multiprotein Systems of Cell Regulation and Signalling: What Can We Learn from NHEJ System of Double-Strand Break Repair?....Pages 1-31
Co-translational Protein Processing, Folding, Targeting, and Membrane Insertion of Newly Synthesized Proteins....Pages 33-41
The Role of Multiple Sequence Repeat Motifs in the Assembly of Multi-protein Complexes....Pages 43-49
Cryoelectron Tomography or Doing Structural Biology In Situ....Pages 51-54
RuvBL1 and RuvBL2 and Their Complex Proteins Implicated in Many Cellular Pathways....Pages 55-63
The Structural Biology of Muscle: Spatial and Temporal Aspects....Pages 65-78
Molecular Basis of Allosteric Transitions: GroEL....Pages 79-86
Cell Signalling Through Covalent Modification and Allostery....Pages 87-92
Combining Cryo-EM and X-ray Crystallography to Study Membrane Protein Structure and Function....Pages 93-101
Molecular Mechanisms of DNA Polymerase Clamp Loaders....Pages 103-114
Electron Microscopy of Macromolecular Machines....Pages 115-123
Assembly and Function of the Signal Recognition Particle from Archaea....Pages 125-133
Structural Studies of the Functional Complexes of the 50S and 70S Ribosome, a Major Antibiotic Target....Pages 135-148
Proteopedia : Exciting Advances in the 3D Encyclopedia of Biomolecular Structure....Pages 149-161
Structure Analysis of Biological Macromolecules by Small-Angle X-ray Scattering....Pages 163-171
Structural Dynamics of the Vault Ribonucleoprotein Particle....Pages 173-182
Structural Dynamics of Picornaviral RdRP Complexes. Implications for the Design of Antivirals....Pages 183-193
Ribosomes: Ribozymes that Survived Evolution Pressures but Is Paralyzed by Tiny Antibiotics....Pages 195-208
