Content: Phospholipase A[subscript]1 assays using a radiolabeled substrate and mass spectrometry / R. Morikawa ... [et al.] -- Real-time cell assays of phospholipase A[subscript]2s using fluorogenic phospholipids / D. Manna and W. Cho -- Analysis and pharmacological targeting of phospholipase C [beta] interactions with G proteins / D.M. Lehmann ... [et al.] -- Biochemical analysis of phospholipase D / H.A. Brown ... [et al.] -- Measurement of autotoxin / lysophospholipase d activity / A.J. Morris and S.S. Smyth -- Platelet-activating factor / J.S. Owen ... [et al.] -- Quantitative measurement of phosphatidylinositol 3,4,5-trisphosphate / H. Guillou ... [et al.] -- Measuring phosphorylated Akt and other phosphoinositide 3-kinase-regulated phosphoproteins in primary lymphocytes / A.C. Donahue ... [et al.] -- Regulation of phosphatidylinositol 4-phosphate 5-kinase activity by partner proteins / Y. Kanaho ... [et al.] -- Biochemical analysis of inositol phosphate kinases / J.C. Otto ... [et al.] -- Analysis of phosphoinositides and their aqueous metabolites / C.P. Berrie ... [et al.] -- Combination of C[subscript]17 sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism / S. Spassieva ... [et al.] -- Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo / M. Maceyka ... [et al.] -- A rapid and sensitive method to measure secretion of sphingosine-1-phosphate / P. Mitra ... [et al.] -- Ceramide kinase and ceramide-1-phosphate / D.S. Wijesinghe ... [et al.] -- Measurement of mammalian diacylglycerol kinase activity in vitro and in cells / R.M. Epand and M.K. Topham -- Lipid phosphate phosphatases from Saccharomyces cerevisiae / G.M. Carman and W.I. Wu
Author(s): Pierre Meystre, Murray Sargent
Series: Methods in Enzymology 434
Publisher: Academic Press
Year: 2007
Language: English
Pages: 387