This textbook introduces to the fundamentals and applications of insect biotechnology in a concise manner. Global economically important insects such as silkworm, lac or honey bee are in the focus of this text. The book attempts to address all of the latest developments in this growing field such as application of metagenomics and proteomics in mining for genes and enzymes that could be beneficial in diverse industrial and biomedical applications. Further, insects have been established as suitable tool for the production of transgenic products which is discussed in one dedicated chapter.
The book targets students and researchers in molecular biology, biotechnology or entomology who want to get familiar with the emerging field of insect biotechnology.
Author(s): Dhiraj Kumar, Sangeeta Shukla
Series: Learning Materials in Biosciences
Publisher: Springer
Year: 2023
Language: English
Pages: 143
City: Cham
Preface
Acknowledgement
Contents
About the Editors
1: Introduction and History of Insect Biotechnology
What You Will Learn from This Chapter
1.1 Introduction
1.2 Insect Evolution
1.3 History of Insect Biotechnology
1.3.1 Role of Biotechnology in Insect Diseases Diagnosis
1.4 Insect Biotechnology and Agriculture
1.5 RNA Interference Mechanism in Insects
1.6 Insects Role in Molecular Medicines
1.7 Conclusion
Take Home Message
References
2: Why Biotechnology Needed in Insects?
What You Will Learn from This Chapter
2.1 Introduction
2.2 OMICS Tools in Insect Science
2.2.1 Genomics
2.2.2 Transcriptomics
2.2.3 Proteomics
2.2.4 Metabolomics
2.3 Biotechnological Interventions Leading to Disease Control in Insects: Silkworm as a Model Organism
2.4 Identification of Silkworm Pathogens
2.4.1 Bacteria
2.4.2 Fungi
2.4.3 Microsporidia
2.4.4 Viruses
2.5 Development of Disease-Resistant/Tolerant Silkworm Breeds
2.6 Novel Products from Insect Origin with Therapeutic Potential
2.7 Conclusion
Take Home Message
References
3: Importance and Application of Biotechnology in Insects
What You Will Learn from This Chapter
3.1 Introduction
3.2 Insect Products with Biomedical Uses
3.3 Problematic Insects
3.3.1 Biotechnological Applications in Insect Pest Control
3.4 Conclusion
Take Home Message
References
4: Recent Advances in Insect Biotechnology
What You Will Learn from This Chapter
4.1 Historical Prospective and Introduction
4.2 Advances of Insect Biotechnology in Agriculture Sector
4.2.1 Bacillus thuringiensis (Bt) and Their Management in Crops
4.2.2 Role of Molecular Markers in the Agriculture Field
4.2.3 Integrated Pest Management (IPM) for Plants and Crops
4.2.4 Biopesticide Development and Crop Resistance
4.3 Advances of Insect Biotechnology in the Health Care Sector
4.3.1 Insect Cell Culture and Drug Discovery
4.4 Advances of Insect Biotechnology in the Industrial Production Sector
4.4.1 Insect Cell Cultures (ICCs)for Large Scale Production
4.4.2 Insect Cell Baculovirus Expression Vector (IC-BEVS) Development and Its Use
4.5 Conclusion
Take Home Message
References
Further Readings
5: Insect Metagenomics
What You Will Learn from This Chapter
5.1 Introduction
5.2 Methods of Insect Metagenomic Analysis
5.2.1 Denaturing Gradient Gel Electrophoresis (DGGE)
5.2.2 Fluorescent In Situ Hybridization (FISH)
5.2.2.1 Sample Preparation
5.2.2.2 Fixation
5.2.2.3 Hybridization
5.2.3 Next-Generation Sequencing (NGS)
5.2.3.1 Sample Collection
5.2.3.2 DNA Extraction and Sample Preparation for NGS
5.2.3.3 Bioinformatic Analysis of Raw Data
5.2.3.4 Metagenome Annotation
5.2.3.5 16S rRNA Sequencing and Taxonomic Analysis
5.3 Applications of Insect Gut Microbiome
5.3.1 Various Industrially Important Enzymes
5.3.2 Signaling Molecules
5.3.3 Health Nutrients
5.3.4 Antibiotic Resistance Gene
5.3.5 Nitrogen Fixation
Take Home Message
References
6: Molecular Signatures of Host-Pathogen Interactions in Virus-Infected Lepidopterans
What You Will Learn from This Chapter
6.1 Introduction
6.2 Insect Antiviral Immune Pathways
6.3 RNAi Antiviral Response
6.4 Nf-kappaB Innate Immune Pathways
6.5 JAK/STAT Pathway
6.6 Virus-Modulated Host Signaling Pathways
6.6.1 PPO Pathway
6.6.2 PI3K/Akt Pathway
6.6.3 ERK Pathway
6.7 Autophagy
6.8 Antimicrobial Peptides (AMPs)
6.9 Apoptosis and Global Protein Synthesis Shutdown
6.10 Others Factors Involved in Antiviral Immunity
6.11 Conclusion
Take Home Message
References
7: Future Prospects of Insect Biotechnology
What You Will Learn from This Chapter
7.1 Introduction and Scope
7.2 Protein Vaccines Produced in Insect Cells (BEVS-Baculovirus Insect Cell Expression System)
7.3 Self-Limiting Agricultural Insect Pests
7.4 A Biological Approach to Pest Management Using Genetically Engineered Insects
7.5 Biorational Pesticide
7.6 Identification of Specific Pest Control Molecules
7.7 Recent Biotechnological Advances in Forest Pest Management
7.8 Entomopathogenic Fungi
7.9 Summary
Take Home Message
References
Index
