Imaging Cellular and Molecular Biological Function provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.
Author(s): Spencer L. Shorte (Editor), Friedrich Frischknecht (Editor)
Edition: 1
Year: 2007
Language: English
Pages: 450
Tags: Биологические дисциплины;
Cover......Page 1
Imaging Cellular and
Molecular Biological
Functions......Page 3
Principles and Practice......Page 2
ISBN-10 3540713301......Page 4
Preface......Page 5
Contents......Page 8
Contributors......Page 16
I
Considerations for Routine Imaging......Page 21
1
Entering the Portal:
Understanding the Digital Image
Recorded Through a Microscope......Page 22
2
Quantitative Biological Image Analysis......Page 63
3
The Open Microscopy Environment:
A Collaborative Data Modeling and Software
Development Project for Biological......Page 89
4
Design and Function of a Light-Microscopy
Facility......Page 111
II
Advanced Methods and Concepts......Page 132
5
Quantitative Colocalisation Imaging: Concepts,
Measurements, and Pitfalls......Page 133
6
Quantitative FRET Microscopy of Live Cells......Page 172
7
Fluorescence Photobleaching and Fluorescence
Correlation Spectroscopy: Two Complementary
Technologies To Study Molecular Dynamics in
Living Cells......Page 197
8
Single Fluorescent Molecule Tracking
in Live Cells......Page 248
9
From Live-Cell Microscopy to Molecular
Mechanisms: Deciphering the Functions
of Kinetochore Proteins......Page 277
III
Cutting Edge Applications & Utilities......Page 298
10
Towards Imaging the Dynamics of Protein
Signalling......Page 299
11
New Technologies for Imaging and Analysis
of Individual Microbial Cells......Page 323
12
Imaging Parasites in Vivo......Page 354
13
Computer-Assisted Systems for Dynamic
3D Reconstruction and Motion Analysis
of Living Cells......Page 374
14
High-Throughput/High-Content Automated
Image Acquisition and Analysis......Page 394
15
Cognition Network Technology – A Novel
Multimodal Image Analysis Technique for
Automatic Identification and Quantification
of Biological Image Contents......Page 415
16
High-Content Phenotypic Cell-Based Assays......Page 431
Index......Page 451
